Analysis of single nucleotide polymorphisms (SNPs) associated with antibiotic resistance genes in Chilean Piscirickettsia salmonis strains

Jaime Figueroa, Diana Castro, Fernando Lagos, Carlos Cartes, Adolfo Isla, Alejandro J. Yáñez, Rubén Avendaño-Herrera, Denise Haussmann

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

The aetiological agent of Piscirickettsiosis is Piscirickettsia salmonis, a Gram-negative intracellular pathogen, and high doses of antibiotics have regularly been employed to treat this infection. Seven florfenicol and/or oxytetracycline resistance genes (tet pump, tetE, Tclor/flor, Tbcr, TfloR, ompF and mdtN) were identified in strains by in silico genome analyses. Later, the number of single nucleotide polymorphisms (SNPs) and its relationship with the resistance to these antibiotics were identified and analysed, using the original LF-89 strain as reference. Trials to determine and compare the minimum inhibitory concentration (MIC) of oxytetracycline and florfenicol in each strain, as well as to quantify the gPCR transcripts levels in the selected genes, were performed. Therefore, variations in the resistance to both antibiotics were observed, where the strain with fewer SNPs showed the highest susceptibility. Consistently, the in silico 3D analyses of proteins encoded by the selected genes revealed structural changes, evident in the sequences with the highest number of SNPs. These results showed that the bacterial resistance to oxytetracycline was mainly linked to the presence of SNPs in relevant sites, antibiotic resistance genes and an OmpF porin, leading to important changes in the protein structure.

Idioma originalInglés
Páginas (desde-hasta)1645-1655
Número de páginas11
PublicaciónJournal of Fish Diseases
Volumen42
N.º12
DOI
EstadoPublicada - 1 dic 2019

Áreas temáticas de ASJC Scopus

  • Ciencias acuáticas
  • Veterinaria (miscelánea)

Huella Profundice en los temas de investigación de 'Analysis of single nucleotide polymorphisms (SNPs) associated with antibiotic resistance genes in Chilean Piscirickettsia salmonis strains'. En conjunto forman una huella única.

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