Analysis of single nucleotide polymorphisms (SNPs) associated with antibiotic resistance genes in Chilean Piscirickettsia salmonis strains

Jaime Figueroa, Diana Castro, Fernando Lagos, Carlos Cartes, Adolfo Isla, Alejandro J. Yáñez, Rubén Avendaño-Herrera, Denise Haussmann

Resultado de la investigación: Article

Resumen

The aetiological agent of Piscirickettsiosis is Piscirickettsia salmonis, a Gram-negative intracellular pathogen, and high doses of antibiotics have regularly been employed to treat this infection. Seven florfenicol and/or oxytetracycline resistance genes (tet pump, tetE, Tclor/flor, Tbcr, TfloR, ompF and mdtN) were identified in strains by in silico genome analyses. Later, the number of single nucleotide polymorphisms (SNPs) and its relationship with the resistance to these antibiotics were identified and analysed, using the original LF-89 strain as reference. Trials to determine and compare the minimum inhibitory concentration (MIC) of oxytetracycline and florfenicol in each strain, as well as to quantify the gPCR transcripts levels in the selected genes, were performed. Therefore, variations in the resistance to both antibiotics were observed, where the strain with fewer SNPs showed the highest susceptibility. Consistently, the in silico 3D analyses of proteins encoded by the selected genes revealed structural changes, evident in the sequences with the highest number of SNPs. These results showed that the bacterial resistance to oxytetracycline was mainly linked to the presence of SNPs in relevant sites, antibiotic resistance genes and an OmpF porin, leading to important changes in the protein structure.

Idioma originalEnglish
PublicaciónJournal of Fish Diseases
DOI
EstadoAccepted/In press - 1 ene 2019

Huella dactilar

Piscirickettsia
Piscirickettsia salmonis
antibiotic resistance
Microbial Drug Resistance
Oxytetracycline
oxytetracycline
single nucleotide polymorphism
Single Nucleotide Polymorphism
polymorphism
antibiotics
florfenicol
gene
Piscirickettsiaceae Infections
Computer Simulation
Genes
genes
Anti-Bacterial Agents
porins
Porins
protein

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Citar esto

Figueroa, Jaime ; Castro, Diana ; Lagos, Fernando ; Cartes, Carlos ; Isla, Adolfo ; Yáñez, Alejandro J. ; Avendaño-Herrera, Rubén ; Haussmann, Denise. / Analysis of single nucleotide polymorphisms (SNPs) associated with antibiotic resistance genes in Chilean Piscirickettsia salmonis strains. En: Journal of Fish Diseases. 2019.
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Analysis of single nucleotide polymorphisms (SNPs) associated with antibiotic resistance genes in Chilean Piscirickettsia salmonis strains. / Figueroa, Jaime; Castro, Diana; Lagos, Fernando; Cartes, Carlos; Isla, Adolfo; Yáñez, Alejandro J.; Avendaño-Herrera, Rubén; Haussmann, Denise.

En: Journal of Fish Diseases, 01.01.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Analysis of single nucleotide polymorphisms (SNPs) associated with antibiotic resistance genes in Chilean Piscirickettsia salmonis strains

AU - Figueroa, Jaime

AU - Castro, Diana

AU - Lagos, Fernando

AU - Cartes, Carlos

AU - Isla, Adolfo

AU - Yáñez, Alejandro J.

AU - Avendaño-Herrera, Rubén

AU - Haussmann, Denise

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The aetiological agent of Piscirickettsiosis is Piscirickettsia salmonis, a Gram-negative intracellular pathogen, and high doses of antibiotics have regularly been employed to treat this infection. Seven florfenicol and/or oxytetracycline resistance genes (tet pump, tetE, Tclor/flor, Tbcr, TfloR, ompF and mdtN) were identified in strains by in silico genome analyses. Later, the number of single nucleotide polymorphisms (SNPs) and its relationship with the resistance to these antibiotics were identified and analysed, using the original LF-89 strain as reference. Trials to determine and compare the minimum inhibitory concentration (MIC) of oxytetracycline and florfenicol in each strain, as well as to quantify the gPCR transcripts levels in the selected genes, were performed. Therefore, variations in the resistance to both antibiotics were observed, where the strain with fewer SNPs showed the highest susceptibility. Consistently, the in silico 3D analyses of proteins encoded by the selected genes revealed structural changes, evident in the sequences with the highest number of SNPs. These results showed that the bacterial resistance to oxytetracycline was mainly linked to the presence of SNPs in relevant sites, antibiotic resistance genes and an OmpF porin, leading to important changes in the protein structure.

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KW - oxytetracycline

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KW - resistance genes

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