Analysis of protease activity in live antigen-presenting cells shows regulation of the phagosomal proteolytic contents during dendritic cell activation

Ana Maria Lennon-Duménil, Arnold H. Bakker, René Maehr, Edda Fiebiger, Herman S. Overkleeft, Mario Rosemblatt, Hidde L. Ploegh, Cécile Lagaudrière-Gesbert

Resultado de la investigación: Article

159 Citas (Scopus)

Resumen

Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow-derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte.

Idioma originalEnglish
Páginas (desde-hasta)529-539
Número de páginas11
PublicaciónJournal of Experimental Medicine
Volumen196
N.º4
DOI
EstadoPublished - 19 ago 2002

Huella dactilar

Phagosomes
Antigen-Presenting Cells
Dendritic Cells
Peptide Hydrolases
cathepsin S
Macrophages
Phagocytes
Lipopolysaccharides
Epitopes
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Citar esto

Lennon-Duménil, Ana Maria ; Bakker, Arnold H. ; Maehr, René ; Fiebiger, Edda ; Overkleeft, Herman S. ; Rosemblatt, Mario ; Ploegh, Hidde L. ; Lagaudrière-Gesbert, Cécile. / Analysis of protease activity in live antigen-presenting cells shows regulation of the phagosomal proteolytic contents during dendritic cell activation. En: Journal of Experimental Medicine. 2002 ; Vol. 196, N.º 4. pp. 529-539.
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abstract = "Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow-derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte.",
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Analysis of protease activity in live antigen-presenting cells shows regulation of the phagosomal proteolytic contents during dendritic cell activation. / Lennon-Duménil, Ana Maria; Bakker, Arnold H.; Maehr, René; Fiebiger, Edda; Overkleeft, Herman S.; Rosemblatt, Mario; Ploegh, Hidde L.; Lagaudrière-Gesbert, Cécile.

En: Journal of Experimental Medicine, Vol. 196, N.º 4, 19.08.2002, p. 529-539.

Resultado de la investigación: Article

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AU - Lennon-Duménil, Ana Maria

AU - Bakker, Arnold H.

AU - Maehr, René

AU - Fiebiger, Edda

AU - Overkleeft, Herman S.

AU - Rosemblatt, Mario

AU - Ploegh, Hidde L.

AU - Lagaudrière-Gesbert, Cécile

PY - 2002/8/19

Y1 - 2002/8/19

N2 - Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow-derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte.

AB - Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow-derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte.

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