TY - GEN
T1 - Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation
AU - Salazar, Camila N.
AU - Acosta, Mauricio
AU - Galleguillos, Pedro A.
AU - Shmaryahu, Amir
AU - Quatrini, Raquel
AU - Holmes, David S.
AU - Demergasso, Cecilia S.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - Acidithiobacillus ferrooxidans strain D2 was isolated from a copper bioleaching operation in Atacama Desert, Chile. Copper is widely used as cofactor in proteins but high concentrations of copper are toxic. Cells require certain mechanisms to maintain the copper homeostasis and avoid toxic effects of high intracellular concentration. The molecular response of A. ferrooxidans strain D2 grown in the presence/absence of copper was examined using a A. ferrooxidans whole-genome DNA microarrays. Roughly 23% of 3,147 genes represented on the microarray were differentially expressed; about 9% of them were upregulated in the presence of copper. Among the upregulated genes, those encoding for the copper efflux protein (CusA) and for the copper-translocating P-type ATPase (CopA) were upregulated. The expression of genes encoding proteins related to iron transport was repressed. Similarly, genes related with assimilative metabolism of sulfur (L-cysteine biosynthesis) cysB, cysJ, cysI, CysD-2 and cysN were upregulated. Our results show that when A. ferrooxidans strain D2 was challenged with high copper concentrations, genes related to copper stress response were upregulated as well as others that have not been reported to be related to that mechanism. In addition, some genes related to other metabolic pathways were repressed, probably because of the energy cost of the stress response.
AB - Acidithiobacillus ferrooxidans strain D2 was isolated from a copper bioleaching operation in Atacama Desert, Chile. Copper is widely used as cofactor in proteins but high concentrations of copper are toxic. Cells require certain mechanisms to maintain the copper homeostasis and avoid toxic effects of high intracellular concentration. The molecular response of A. ferrooxidans strain D2 grown in the presence/absence of copper was examined using a A. ferrooxidans whole-genome DNA microarrays. Roughly 23% of 3,147 genes represented on the microarray were differentially expressed; about 9% of them were upregulated in the presence of copper. Among the upregulated genes, those encoding for the copper efflux protein (CusA) and for the copper-translocating P-type ATPase (CopA) were upregulated. The expression of genes encoding proteins related to iron transport was repressed. Similarly, genes related with assimilative metabolism of sulfur (L-cysteine biosynthesis) cysB, cysJ, cysI, CysD-2 and cysN were upregulated. Our results show that when A. ferrooxidans strain D2 was challenged with high copper concentrations, genes related to copper stress response were upregulated as well as others that have not been reported to be related to that mechanism. In addition, some genes related to other metabolic pathways were repressed, probably because of the energy cost of the stress response.
KW - At. ferrooxidans
KW - Copper
KW - Microarray
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=84886777520&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.825.157
DO - 10.4028/www.scientific.net/AMR.825.157
M3 - Conference contribution
AN - SCOPUS:84886777520
SN - 9783037858912
T3 - Advanced Materials Research
SP - 157
EP - 161
BT - Integration of Scientific and Industrial Knowledge on Biohydrometallurgy
T2 - 20th International Biohydrometallurgy Symposium, IBS 2013
Y2 - 8 October 2013 through 11 October 2013
ER -