Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation

Camila N. Salazar, Mauricio Acosta, Pedro A. Galleguillos, Amir Shmaryahu, Raquel Quatrini, David S. Holmes, Cecilia S. Demergasso

Resultado de la investigación: Conference contribution

7 Citas (Scopus)

Resumen

Acidithiobacillus ferrooxidans strain D2 was isolated from a copper bioleaching operation in Atacama Desert, Chile. Copper is widely used as cofactor in proteins but high concentrations of copper are toxic. Cells require certain mechanisms to maintain the copper homeostasis and avoid toxic effects of high intracellular concentration. The molecular response of A. ferrooxidans strain D2 grown in the presence/absence of copper was examined using a A. ferrooxidans whole-genome DNA microarrays. Roughly 23% of 3,147 genes represented on the microarray were differentially expressed; about 9% of them were upregulated in the presence of copper. Among the upregulated genes, those encoding for the copper efflux protein (CusA) and for the copper-translocating P-type ATPase (CopA) were upregulated. The expression of genes encoding proteins related to iron transport was repressed. Similarly, genes related with assimilative metabolism of sulfur (L-cysteine biosynthesis) cysB, cysJ, cysI, CysD-2 and cysN were upregulated. Our results show that when A. ferrooxidans strain D2 was challenged with high copper concentrations, genes related to copper stress response were upregulated as well as others that have not been reported to be related to that mechanism. In addition, some genes related to other metabolic pathways were repressed, probably because of the energy cost of the stress response.

Idioma originalEnglish
Título de la publicación alojadaIntegration of Scientific and Industrial Knowledge on Biohydrometallurgy
Páginas157-161
Número de páginas5
Volumen825
DOI
EstadoPublished - 2013
Evento20th International Biohydrometallurgy Symposium, IBS 2013 - Antofagasta, Chile
Duración: 8 oct 201311 oct 2013

Serie de la publicación

NombreAdvanced Materials Research
Volumen825
ISSN (versión impresa)10226680

Other

Other20th International Biohydrometallurgy Symposium, IBS 2013
PaísChile
CiudadAntofagasta
Período8/10/1311/10/13

Huella dactilar

Bioleaching
Gene expression
Copper
Genes
Gene encoding
Microarrays
Proteins
Biosynthesis
Metabolism
DNA
Sulfur
Iron

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Salazar, C. N., Acosta, M., Galleguillos, P. A., Shmaryahu, A., Quatrini, R., Holmes, D. S., & Demergasso, C. S. (2013). Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation. En Integration of Scientific and Industrial Knowledge on Biohydrometallurgy (Vol. 825, pp. 157-161). (Advanced Materials Research; Vol. 825). https://doi.org/10.4028/www.scientific.net/AMR.825.157
Salazar, Camila N. ; Acosta, Mauricio ; Galleguillos, Pedro A. ; Shmaryahu, Amir ; Quatrini, Raquel ; Holmes, David S. ; Demergasso, Cecilia S. / Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation. Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. Vol. 825 2013. pp. 157-161 (Advanced Materials Research).
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abstract = "Acidithiobacillus ferrooxidans strain D2 was isolated from a copper bioleaching operation in Atacama Desert, Chile. Copper is widely used as cofactor in proteins but high concentrations of copper are toxic. Cells require certain mechanisms to maintain the copper homeostasis and avoid toxic effects of high intracellular concentration. The molecular response of A. ferrooxidans strain D2 grown in the presence/absence of copper was examined using a A. ferrooxidans whole-genome DNA microarrays. Roughly 23{\%} of 3,147 genes represented on the microarray were differentially expressed; about 9{\%} of them were upregulated in the presence of copper. Among the upregulated genes, those encoding for the copper efflux protein (CusA) and for the copper-translocating P-type ATPase (CopA) were upregulated. The expression of genes encoding proteins related to iron transport was repressed. Similarly, genes related with assimilative metabolism of sulfur (L-cysteine biosynthesis) cysB, cysJ, cysI, CysD-2 and cysN were upregulated. Our results show that when A. ferrooxidans strain D2 was challenged with high copper concentrations, genes related to copper stress response were upregulated as well as others that have not been reported to be related to that mechanism. In addition, some genes related to other metabolic pathways were repressed, probably because of the energy cost of the stress response.",
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Salazar, CN, Acosta, M, Galleguillos, PA, Shmaryahu, A, Quatrini, R, Holmes, DS & Demergasso, CS 2013, Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation. En Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. vol. 825, Advanced Materials Research, vol. 825, pp. 157-161, 20th International Biohydrometallurgy Symposium, IBS 2013, Antofagasta, Chile, 8/10/13. https://doi.org/10.4028/www.scientific.net/AMR.825.157

Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation. / Salazar, Camila N.; Acosta, Mauricio; Galleguillos, Pedro A.; Shmaryahu, Amir; Quatrini, Raquel; Holmes, David S.; Demergasso, Cecilia S.

Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. Vol. 825 2013. p. 157-161 (Advanced Materials Research; Vol. 825).

Resultado de la investigación: Conference contribution

TY - GEN

T1 - Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation

AU - Salazar, Camila N.

AU - Acosta, Mauricio

AU - Galleguillos, Pedro A.

AU - Shmaryahu, Amir

AU - Quatrini, Raquel

AU - Holmes, David S.

AU - Demergasso, Cecilia S.

PY - 2013

Y1 - 2013

N2 - Acidithiobacillus ferrooxidans strain D2 was isolated from a copper bioleaching operation in Atacama Desert, Chile. Copper is widely used as cofactor in proteins but high concentrations of copper are toxic. Cells require certain mechanisms to maintain the copper homeostasis and avoid toxic effects of high intracellular concentration. The molecular response of A. ferrooxidans strain D2 grown in the presence/absence of copper was examined using a A. ferrooxidans whole-genome DNA microarrays. Roughly 23% of 3,147 genes represented on the microarray were differentially expressed; about 9% of them were upregulated in the presence of copper. Among the upregulated genes, those encoding for the copper efflux protein (CusA) and for the copper-translocating P-type ATPase (CopA) were upregulated. The expression of genes encoding proteins related to iron transport was repressed. Similarly, genes related with assimilative metabolism of sulfur (L-cysteine biosynthesis) cysB, cysJ, cysI, CysD-2 and cysN were upregulated. Our results show that when A. ferrooxidans strain D2 was challenged with high copper concentrations, genes related to copper stress response were upregulated as well as others that have not been reported to be related to that mechanism. In addition, some genes related to other metabolic pathways were repressed, probably because of the energy cost of the stress response.

AB - Acidithiobacillus ferrooxidans strain D2 was isolated from a copper bioleaching operation in Atacama Desert, Chile. Copper is widely used as cofactor in proteins but high concentrations of copper are toxic. Cells require certain mechanisms to maintain the copper homeostasis and avoid toxic effects of high intracellular concentration. The molecular response of A. ferrooxidans strain D2 grown in the presence/absence of copper was examined using a A. ferrooxidans whole-genome DNA microarrays. Roughly 23% of 3,147 genes represented on the microarray were differentially expressed; about 9% of them were upregulated in the presence of copper. Among the upregulated genes, those encoding for the copper efflux protein (CusA) and for the copper-translocating P-type ATPase (CopA) were upregulated. The expression of genes encoding proteins related to iron transport was repressed. Similarly, genes related with assimilative metabolism of sulfur (L-cysteine biosynthesis) cysB, cysJ, cysI, CysD-2 and cysN were upregulated. Our results show that when A. ferrooxidans strain D2 was challenged with high copper concentrations, genes related to copper stress response were upregulated as well as others that have not been reported to be related to that mechanism. In addition, some genes related to other metabolic pathways were repressed, probably because of the energy cost of the stress response.

KW - At. ferrooxidans

KW - Copper

KW - Microarray

KW - Stress

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U2 - 10.4028/www.scientific.net/AMR.825.157

DO - 10.4028/www.scientific.net/AMR.825.157

M3 - Conference contribution

AN - SCOPUS:84886777520

SN - 9783037858912

VL - 825

T3 - Advanced Materials Research

SP - 157

EP - 161

BT - Integration of Scientific and Industrial Knowledge on Biohydrometallurgy

ER -

Salazar CN, Acosta M, Galleguillos PA, Shmaryahu A, Quatrini R, Holmes DS y otros. Analysis of gene expression in response to copper stress in Acidithiobacillus ferrooxidans strain D2, isolated from a copper bioleaching operation. En Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. Vol. 825. 2013. p. 157-161. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.825.157