An increased expression of nucleolin is associated with a physiological nucleolar segregation

M. Alvarez, C. Quezada, C. Navarro, A. Molina, P. Bouvet, M. Krauskopf, M. I. Vera

Resultado de la investigación: Article

22 Citas (Scopus)

Resumen

Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis.

Idioma originalEnglish
Páginas (desde-hasta)152-158
Número de páginas7
PublicaciónBiochemical and Biophysical Research Communications
Volumen301
N.º1
DOI
EstadoPublished - 31 ene 2003

Huella dactilar

Carps
Nuclear Proteins
Fish
Fishes
Physiology
Transcription
Liver
Acclimatization
Ribosomes
Complementary DNA
Genes
Amino Acid Sequence
Amino Acids
Up-Regulation
Messenger RNA
Immunohistochemistry
nucleolin
Processing

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Alvarez, M. ; Quezada, C. ; Navarro, C. ; Molina, A. ; Bouvet, P. ; Krauskopf, M. ; Vera, M. I. / An increased expression of nucleolin is associated with a physiological nucleolar segregation. En: Biochemical and Biophysical Research Communications. 2003 ; Vol. 301, N.º 1. pp. 152-158.
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An increased expression of nucleolin is associated with a physiological nucleolar segregation. / Alvarez, M.; Quezada, C.; Navarro, C.; Molina, A.; Bouvet, P.; Krauskopf, M.; Vera, M. I.

En: Biochemical and Biophysical Research Communications, Vol. 301, N.º 1, 31.01.2003, p. 152-158.

Resultado de la investigación: Article

TY - JOUR

T1 - An increased expression of nucleolin is associated with a physiological nucleolar segregation

AU - Alvarez, M.

AU - Quezada, C.

AU - Navarro, C.

AU - Molina, A.

AU - Bouvet, P.

AU - Krauskopf, M.

AU - Vera, M. I.

PY - 2003/1/31

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AB - Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis.

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KW - Fish

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KW - U3 snoRNA

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