Alleviation of metal stress by Pseudomonas orientalis and Chaetomium cupreum strains and their effects on Eucalyptus globulus growth promotion

J. Ortiz, J. Soto, L. Almonacid, A. Fuentes, R. Campos-Vargas, C. Arriagada

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Aim: Pseudomonas orientalis and Chaetomium cupreum isolated from metal(oid)s-contaminated soil were characterized to evaluate their role in plant growth promotion and enhance the tolerance of Eucalyptus globulus to copper stress. Methods: We evaluated minimum inhibitory concentration and explored plant-promoting traits under copper stress. Moreover, changes in morphology and the accumulation of metals on the cell surface were determined by scanning electron microscopy images coupled with energy dispersive x-ray spectroscopy (SEM-EDX). The effect of inoculation with P. orientalis and C. cupreum on E. globulus plants established in soils contaminated with copper was determined through root and shoot biomass and antioxidant response. Results: Both strains showed high tolerance to metal(oid)s, whereas phosphate solubilization was only detected in P. orientalis. Under copper stress, C. cupreum presented a higher production rate of siderophores and indole acetic acid (4.5 mm day−1, 15.042 μg mL−1). SEM images and EDX analysis reflected cellular changes in fungus and proved biosorption of copper. Inoculation of P. orientalis and C. cupreum on Eucalyptus globulus plants significantly increased plant growth and mitigated the toxic effects of copper. Conclusions: According to these results, both microorganisms contribute positively to stimulated plant growth and to decreasing stress caused by high concentrations of copper in soil, especially C. cupreum.

Idioma originalEnglish
PublicaciónPlant and Soil
DOI
EstadoAccepted/In press - 1 ene 2019

Huella dactilar

Pseudomonas orientalis
Chaetomium
growth promotion
Eucalyptus globulus
copper
metals
metal
scanning electron microscopy
plant growth
polluted soils
inoculation
tolerance
biosorption
siderophore
siderophores
solubilization
minimum inhibitory concentration
effect
acetic acid
indole acetic acid

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Citar esto

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title = "Alleviation of metal stress by Pseudomonas orientalis and Chaetomium cupreum strains and their effects on Eucalyptus globulus growth promotion",
abstract = "Aim: Pseudomonas orientalis and Chaetomium cupreum isolated from metal(oid)s-contaminated soil were characterized to evaluate their role in plant growth promotion and enhance the tolerance of Eucalyptus globulus to copper stress. Methods: We evaluated minimum inhibitory concentration and explored plant-promoting traits under copper stress. Moreover, changes in morphology and the accumulation of metals on the cell surface were determined by scanning electron microscopy images coupled with energy dispersive x-ray spectroscopy (SEM-EDX). The effect of inoculation with P. orientalis and C. cupreum on E. globulus plants established in soils contaminated with copper was determined through root and shoot biomass and antioxidant response. Results: Both strains showed high tolerance to metal(oid)s, whereas phosphate solubilization was only detected in P. orientalis. Under copper stress, C. cupreum presented a higher production rate of siderophores and indole acetic acid (4.5 mm day−1, 15.042 μg mL−1). SEM images and EDX analysis reflected cellular changes in fungus and proved biosorption of copper. Inoculation of P. orientalis and C. cupreum on Eucalyptus globulus plants significantly increased plant growth and mitigated the toxic effects of copper. Conclusions: According to these results, both microorganisms contribute positively to stimulated plant growth and to decreasing stress caused by high concentrations of copper in soil, especially C. cupreum.",
keywords = "Metal stress, Phytoremediation, Plant growth promotion, Soil microorganisms",
author = "J. Ortiz and J. Soto and L. Almonacid and A. Fuentes and R. Campos-Vargas and C. Arriagada",
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Alleviation of metal stress by Pseudomonas orientalis and Chaetomium cupreum strains and their effects on Eucalyptus globulus growth promotion. / Ortiz, J.; Soto, J.; Almonacid, L.; Fuentes, A.; Campos-Vargas, R.; Arriagada, C.

En: Plant and Soil, 01.01.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Alleviation of metal stress by Pseudomonas orientalis and Chaetomium cupreum strains and their effects on Eucalyptus globulus growth promotion

AU - Ortiz, J.

AU - Soto, J.

AU - Almonacid, L.

AU - Fuentes, A.

AU - Campos-Vargas, R.

AU - Arriagada, C.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Aim: Pseudomonas orientalis and Chaetomium cupreum isolated from metal(oid)s-contaminated soil were characterized to evaluate their role in plant growth promotion and enhance the tolerance of Eucalyptus globulus to copper stress. Methods: We evaluated minimum inhibitory concentration and explored plant-promoting traits under copper stress. Moreover, changes in morphology and the accumulation of metals on the cell surface were determined by scanning electron microscopy images coupled with energy dispersive x-ray spectroscopy (SEM-EDX). The effect of inoculation with P. orientalis and C. cupreum on E. globulus plants established in soils contaminated with copper was determined through root and shoot biomass and antioxidant response. Results: Both strains showed high tolerance to metal(oid)s, whereas phosphate solubilization was only detected in P. orientalis. Under copper stress, C. cupreum presented a higher production rate of siderophores and indole acetic acid (4.5 mm day−1, 15.042 μg mL−1). SEM images and EDX analysis reflected cellular changes in fungus and proved biosorption of copper. Inoculation of P. orientalis and C. cupreum on Eucalyptus globulus plants significantly increased plant growth and mitigated the toxic effects of copper. Conclusions: According to these results, both microorganisms contribute positively to stimulated plant growth and to decreasing stress caused by high concentrations of copper in soil, especially C. cupreum.

AB - Aim: Pseudomonas orientalis and Chaetomium cupreum isolated from metal(oid)s-contaminated soil were characterized to evaluate their role in plant growth promotion and enhance the tolerance of Eucalyptus globulus to copper stress. Methods: We evaluated minimum inhibitory concentration and explored plant-promoting traits under copper stress. Moreover, changes in morphology and the accumulation of metals on the cell surface were determined by scanning electron microscopy images coupled with energy dispersive x-ray spectroscopy (SEM-EDX). The effect of inoculation with P. orientalis and C. cupreum on E. globulus plants established in soils contaminated with copper was determined through root and shoot biomass and antioxidant response. Results: Both strains showed high tolerance to metal(oid)s, whereas phosphate solubilization was only detected in P. orientalis. Under copper stress, C. cupreum presented a higher production rate of siderophores and indole acetic acid (4.5 mm day−1, 15.042 μg mL−1). SEM images and EDX analysis reflected cellular changes in fungus and proved biosorption of copper. Inoculation of P. orientalis and C. cupreum on Eucalyptus globulus plants significantly increased plant growth and mitigated the toxic effects of copper. Conclusions: According to these results, both microorganisms contribute positively to stimulated plant growth and to decreasing stress caused by high concentrations of copper in soil, especially C. cupreum.

KW - Metal stress

KW - Phytoremediation

KW - Plant growth promotion

KW - Soil microorganisms

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U2 - 10.1007/s11104-019-03946-w

DO - 10.1007/s11104-019-03946-w

M3 - Article

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

ER -