Adhesion of B cell lines to endothelial cells from human lymphoid tissue modulates tyrosine phosphorylation and endothelial cell activation

Lilian I. Reyes, Paula Escobar, Maria R. Bono, Mario Rosemblatt

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Through the production of cytokines and growth factors the endothelium of secondary lymphoid organs plays a crucial role in controlling lymphocyte migration to the lymphoid microenvironment, an essential step in the initiation of the immune response. Here we demonstrate that direct contact of B cell lines with tonsil-derived human endothelial cells resulted in changes in the phosphorylation state of endothelial cells, causing their functional activation. We found a rapid (<15-s) and transient dephosphorylation, followed by a rapid rephosphorylation of tyrosine residues of the focal adhesion kinase, paxillin, and ERK2. Maximal rephosphorylation occurred after 15-30 min of B cell contact. Preincubation of lymphoid B cells with an adhesion-blocking Ab directed against α4β1 integrin abrogated adhesion-mediated changes of endothelial cell tyrosine phosphorylation, suggesting that cell contact was essential. Similar patterns of tyrosine phosphorylation, but with slightly different kinetics were induced after cross-linking of β1 integrin or CD40 on endothelial cells. Functional activation of endothelial cells by B cell adhesion was confirmed by the production of IL-6, IL-8, monocyte chemoattractant protein-1, M-CSF, and macrophage inflammatory protein-1β mRNA. However, direct cross-linking of β1 integrin and CD40 failed to accomplish the same functional activation. These data indicate that direct contact of lymphoid B cells with the endothelium from lymphoid tissue induce endothelial cell signaling, resulting in chemokine and cytokine production. This phenomenon may provide a mechanism for the remodeling of the endothelium from lymphoid tissues, thus contributing to the free migration of lymphocytes and other cells into the lymphoid organs.

Idioma originalEnglish
Páginas (desde-hasta)5881-5888
Número de páginas8
PublicaciónJournal of Immunology
Volumen169
N.º10
EstadoPublished - 15 nov 2002

Huella dactilar

Lymphoid Tissue
Tyrosine
B-Lymphocytes
Endothelial Cells
Phosphorylation
Cell Line
Lymphocytes
Integrins
Endothelium
Cell Adhesion
Cytokines
Paxillin
Macrophage Inflammatory Proteins
Focal Adhesion Protein-Tyrosine Kinases
Macrophage Colony-Stimulating Factor
Chemokine CCL2
Palatine Tonsil
Interleukin-8
Chemokines
Interleukin-6

ASJC Scopus subject areas

  • Immunology

Citar esto

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abstract = "Through the production of cytokines and growth factors the endothelium of secondary lymphoid organs plays a crucial role in controlling lymphocyte migration to the lymphoid microenvironment, an essential step in the initiation of the immune response. Here we demonstrate that direct contact of B cell lines with tonsil-derived human endothelial cells resulted in changes in the phosphorylation state of endothelial cells, causing their functional activation. We found a rapid (<15-s) and transient dephosphorylation, followed by a rapid rephosphorylation of tyrosine residues of the focal adhesion kinase, paxillin, and ERK2. Maximal rephosphorylation occurred after 15-30 min of B cell contact. Preincubation of lymphoid B cells with an adhesion-blocking Ab directed against α4β1 integrin abrogated adhesion-mediated changes of endothelial cell tyrosine phosphorylation, suggesting that cell contact was essential. Similar patterns of tyrosine phosphorylation, but with slightly different kinetics were induced after cross-linking of β1 integrin or CD40 on endothelial cells. Functional activation of endothelial cells by B cell adhesion was confirmed by the production of IL-6, IL-8, monocyte chemoattractant protein-1, M-CSF, and macrophage inflammatory protein-1β mRNA. However, direct cross-linking of β1 integrin and CD40 failed to accomplish the same functional activation. These data indicate that direct contact of lymphoid B cells with the endothelium from lymphoid tissue induce endothelial cell signaling, resulting in chemokine and cytokine production. This phenomenon may provide a mechanism for the remodeling of the endothelium from lymphoid tissues, thus contributing to the free migration of lymphocytes and other cells into the lymphoid organs.",
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Adhesion of B cell lines to endothelial cells from human lymphoid tissue modulates tyrosine phosphorylation and endothelial cell activation. / Reyes, Lilian I.; Escobar, Paula; Bono, Maria R.; Rosemblatt, Mario.

En: Journal of Immunology, Vol. 169, N.º 10, 15.11.2002, p. 5881-5888.

Resultado de la investigación: Article

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T1 - Adhesion of B cell lines to endothelial cells from human lymphoid tissue modulates tyrosine phosphorylation and endothelial cell activation

AU - Reyes, Lilian I.

AU - Escobar, Paula

AU - Bono, Maria R.

AU - Rosemblatt, Mario

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