Adherence of Clostridium difficile spores to Caco-2 cells in culture

Daniel Paredes-Sabja, Mahfuzur R. Sarker

Resultado de la investigación: Article

40 Citas (Scopus)

Resumen

Clostridium difficile is the causative agent of the majority of antibiotic associated diarrhoea cases. C. difficile spores are recognized as the persistent and infectious morphotype as well as the vehicle of transmission of CDI. However, there is a lack of knowledge on how C. difficile spores interact with the host's epithelial surfaces. In this context, we have characterized the ability of C. difficile spores to adhere to human Caco-2 cells. Despite the similarities in spore-surface hydrophobicity between spores of C. difficile and Clostridium perfringens (another enteric pathogen that also sporulates in the gut), spores of C. difficile adhere better to Caco-2 cells. Adherence to Caco-2 cells was significantly reduced when C. difficile spores were treated with trypsin. Sonication of C. difficile spores altered the ultrastructure of the outermost exosporium-like structure, releasing two protein species of ~40 kDa and significantly reduced spore hydrophobicity and adherence to Caco-2 cells. Using a trifunctional cross-linker, we were able to co-immunoprecipitate four protein species from the surface of Caco-2 cells. In conclusion, this study provides evidence that C. difficile spores adhere to human intestinal enterocyte-like cells through spore- and enterocytic-surface-specific ligand(s) and/or receptor(s).

Idioma originalEnglish
Páginas (desde-hasta)1208-1218
Número de páginas11
PublicaciónJournal of Medical Microbiology
Volumen61
N.ºPART 9
DOI
EstadoPublished - sep 2012

Huella dactilar

Caco-2 Cells
Clostridium difficile
Spores
Cell Culture Techniques
Hydrophobic and Hydrophilic Interactions
Clostridium perfringens
Sonication
Enterocytes
Trypsin
Diarrhea
Membrane Proteins
Anti-Bacterial Agents
Ligands

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Medicine(all)

Citar esto

@article{41a2ddffc6c445c5af4ed1bedbcf413e,
title = "Adherence of Clostridium difficile spores to Caco-2 cells in culture",
abstract = "Clostridium difficile is the causative agent of the majority of antibiotic associated diarrhoea cases. C. difficile spores are recognized as the persistent and infectious morphotype as well as the vehicle of transmission of CDI. However, there is a lack of knowledge on how C. difficile spores interact with the host's epithelial surfaces. In this context, we have characterized the ability of C. difficile spores to adhere to human Caco-2 cells. Despite the similarities in spore-surface hydrophobicity between spores of C. difficile and Clostridium perfringens (another enteric pathogen that also sporulates in the gut), spores of C. difficile adhere better to Caco-2 cells. Adherence to Caco-2 cells was significantly reduced when C. difficile spores were treated with trypsin. Sonication of C. difficile spores altered the ultrastructure of the outermost exosporium-like structure, releasing two protein species of ~40 kDa and significantly reduced spore hydrophobicity and adherence to Caco-2 cells. Using a trifunctional cross-linker, we were able to co-immunoprecipitate four protein species from the surface of Caco-2 cells. In conclusion, this study provides evidence that C. difficile spores adhere to human intestinal enterocyte-like cells through spore- and enterocytic-surface-specific ligand(s) and/or receptor(s).",
author = "Daniel Paredes-Sabja and Sarker, {Mahfuzur R.}",
year = "2012",
month = "9",
doi = "10.1099/jmm.0.043687-0",
language = "English",
volume = "61",
pages = "1208--1218",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "PART 9",

}

Adherence of Clostridium difficile spores to Caco-2 cells in culture. / Paredes-Sabja, Daniel; Sarker, Mahfuzur R.

En: Journal of Medical Microbiology, Vol. 61, N.º PART 9, 09.2012, p. 1208-1218.

Resultado de la investigación: Article

TY - JOUR

T1 - Adherence of Clostridium difficile spores to Caco-2 cells in culture

AU - Paredes-Sabja, Daniel

AU - Sarker, Mahfuzur R.

PY - 2012/9

Y1 - 2012/9

N2 - Clostridium difficile is the causative agent of the majority of antibiotic associated diarrhoea cases. C. difficile spores are recognized as the persistent and infectious morphotype as well as the vehicle of transmission of CDI. However, there is a lack of knowledge on how C. difficile spores interact with the host's epithelial surfaces. In this context, we have characterized the ability of C. difficile spores to adhere to human Caco-2 cells. Despite the similarities in spore-surface hydrophobicity between spores of C. difficile and Clostridium perfringens (another enteric pathogen that also sporulates in the gut), spores of C. difficile adhere better to Caco-2 cells. Adherence to Caco-2 cells was significantly reduced when C. difficile spores were treated with trypsin. Sonication of C. difficile spores altered the ultrastructure of the outermost exosporium-like structure, releasing two protein species of ~40 kDa and significantly reduced spore hydrophobicity and adherence to Caco-2 cells. Using a trifunctional cross-linker, we were able to co-immunoprecipitate four protein species from the surface of Caco-2 cells. In conclusion, this study provides evidence that C. difficile spores adhere to human intestinal enterocyte-like cells through spore- and enterocytic-surface-specific ligand(s) and/or receptor(s).

AB - Clostridium difficile is the causative agent of the majority of antibiotic associated diarrhoea cases. C. difficile spores are recognized as the persistent and infectious morphotype as well as the vehicle of transmission of CDI. However, there is a lack of knowledge on how C. difficile spores interact with the host's epithelial surfaces. In this context, we have characterized the ability of C. difficile spores to adhere to human Caco-2 cells. Despite the similarities in spore-surface hydrophobicity between spores of C. difficile and Clostridium perfringens (another enteric pathogen that also sporulates in the gut), spores of C. difficile adhere better to Caco-2 cells. Adherence to Caco-2 cells was significantly reduced when C. difficile spores were treated with trypsin. Sonication of C. difficile spores altered the ultrastructure of the outermost exosporium-like structure, releasing two protein species of ~40 kDa and significantly reduced spore hydrophobicity and adherence to Caco-2 cells. Using a trifunctional cross-linker, we were able to co-immunoprecipitate four protein species from the surface of Caco-2 cells. In conclusion, this study provides evidence that C. difficile spores adhere to human intestinal enterocyte-like cells through spore- and enterocytic-surface-specific ligand(s) and/or receptor(s).

UR - http://www.scopus.com/inward/record.url?scp=84865170905&partnerID=8YFLogxK

U2 - 10.1099/jmm.0.043687-0

DO - 10.1099/jmm.0.043687-0

M3 - Article

C2 - 22595914

AN - SCOPUS:84865170905

VL - 61

SP - 1208

EP - 1218

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - PART 9

ER -