Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga-Flavobacterium-Bacteroides (CFB) group

Manuel Romero, Rubén Avendaño-Herrera, Beatriz Magariños, Miguel Cámara, Ana Otero

Resultado de la investigación: Article

66 Citas (Scopus)

Resumen

Tenacibaculum maritimum (formerly Flexibacter maritimus) is a filamentous, biofilm-forming member of the Cytophaga-Flavobacterium-Bacteroides group (or Bacteroidetes), which causes the widely distributed marine fish disease tenacibaculosis. A search for N-acylhomoserine lactones (AHLs) quorum-sensing (QS) signals in the culture media of nine representative strains of this species using different biosensor strains revealed the presence of short-type AHL activity in all of them. N-butyryl-l-homoserine lactone (C4-HSL) was identified in T. maritimum NCIMB2154T by LC-MS. A degradation activity for long-acyl AHLs (C10-HSL) was subsequently demonstrated in T. maritimum NCIMB2154T. The acidification of the culture medium after degradation did not allow the recovery of C10-HSL, which indicates a possible acylase-type degradation activity. Even though the physiological processes under the control of AHL-mediated QS in T. maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance.

Idioma originalEnglish
Páginas (desde-hasta)131-139
Número de páginas9
PublicaciónFEMS Microbiology Letters
Volumen304
N.º2
DOI
EstadoPublished - mar 2010

Huella dactilar

Tenacibaculum
Cytophaga
Flavobacterium
Bacteroides
Lactones
Fishes
Quorum Sensing
amidase
Culture Media
Flexibacter
Fish Diseases
Bacteroidetes
Physiological Phenomena
Proteobacteria
Biosensing Techniques
Biofilms

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Citar esto

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abstract = "Tenacibaculum maritimum (formerly Flexibacter maritimus) is a filamentous, biofilm-forming member of the Cytophaga-Flavobacterium-Bacteroides group (or Bacteroidetes), which causes the widely distributed marine fish disease tenacibaculosis. A search for N-acylhomoserine lactones (AHLs) quorum-sensing (QS) signals in the culture media of nine representative strains of this species using different biosensor strains revealed the presence of short-type AHL activity in all of them. N-butyryl-l-homoserine lactone (C4-HSL) was identified in T. maritimum NCIMB2154T by LC-MS. A degradation activity for long-acyl AHLs (C10-HSL) was subsequently demonstrated in T. maritimum NCIMB2154T. The acidification of the culture medium after degradation did not allow the recovery of C10-HSL, which indicates a possible acylase-type degradation activity. Even though the physiological processes under the control of AHL-mediated QS in T. maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance.",
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Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga-Flavobacterium-Bacteroides (CFB) group. / Romero, Manuel; Avendaño-Herrera, Rubén; Magariños, Beatriz; Cámara, Miguel; Otero, Ana.

En: FEMS Microbiology Letters, Vol. 304, N.º 2, 03.2010, p. 131-139.

Resultado de la investigación: Article

TY - JOUR

T1 - Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga-Flavobacterium-Bacteroides (CFB) group

AU - Romero, Manuel

AU - Avendaño-Herrera, Rubén

AU - Magariños, Beatriz

AU - Cámara, Miguel

AU - Otero, Ana

PY - 2010/3

Y1 - 2010/3

N2 - Tenacibaculum maritimum (formerly Flexibacter maritimus) is a filamentous, biofilm-forming member of the Cytophaga-Flavobacterium-Bacteroides group (or Bacteroidetes), which causes the widely distributed marine fish disease tenacibaculosis. A search for N-acylhomoserine lactones (AHLs) quorum-sensing (QS) signals in the culture media of nine representative strains of this species using different biosensor strains revealed the presence of short-type AHL activity in all of them. N-butyryl-l-homoserine lactone (C4-HSL) was identified in T. maritimum NCIMB2154T by LC-MS. A degradation activity for long-acyl AHLs (C10-HSL) was subsequently demonstrated in T. maritimum NCIMB2154T. The acidification of the culture medium after degradation did not allow the recovery of C10-HSL, which indicates a possible acylase-type degradation activity. Even though the physiological processes under the control of AHL-mediated QS in T. maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance.

AB - Tenacibaculum maritimum (formerly Flexibacter maritimus) is a filamentous, biofilm-forming member of the Cytophaga-Flavobacterium-Bacteroides group (or Bacteroidetes), which causes the widely distributed marine fish disease tenacibaculosis. A search for N-acylhomoserine lactones (AHLs) quorum-sensing (QS) signals in the culture media of nine representative strains of this species using different biosensor strains revealed the presence of short-type AHL activity in all of them. N-butyryl-l-homoserine lactone (C4-HSL) was identified in T. maritimum NCIMB2154T by LC-MS. A degradation activity for long-acyl AHLs (C10-HSL) was subsequently demonstrated in T. maritimum NCIMB2154T. The acidification of the culture medium after degradation did not allow the recovery of C10-HSL, which indicates a possible acylase-type degradation activity. Even though the physiological processes under the control of AHL-mediated QS in T. maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance.

KW - Acylhomoserine lactone

KW - Cytophaga- Flavobacterium-Bacteroides

KW - Quorum quenching

KW - Quorum sensing

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