Activity stabilization of Aspergillus niger and Escherichia coli phytases immobilized on allophanic synthetic compounds and montmorillonite nanoclays

Daniel Menezes-Blackburn, Milko Jorquera, Liliana Gianfreda, Maria Rao, Ralf Greiner, Elizabeth Garrido, María De la Luz Mora

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

50 Citas (Scopus)

Resumen

The aim of this work was to study the stabilization of the activity of two commercial microbial phytases (Aspergillus niger and Escherichia coli) after immobilization on nanoclays and to establish optimal conditions for their immobilization. Synthetic allophane, synthetic iron-coated allophanes and natural montmorillonite were chosen as solid supports for phytase immobilization. Phytase immobilization patterns at different pH values were strongly dependent on both enzyme and support characteristics. After immobilization, the residual activity of both phytases was higher under acidic conditions. Immobilization of phytases increased their thermal stability and improved resistance to proteolysis, particularly on iron-coated allophane (6% iron oxide), which showed activation energy (E a) and activation enthalpy (ΔH #) similar to free enzymes. Montmorillonite as well as allophanic synthetic compounds resulted in a good support for immobilization of E. coli phytase, but caused a severe reduction of A. niger phytase activity.

Idioma originalInglés
Páginas (desde-hasta)9360-9367
Número de páginas8
PublicaciónBioresource Technology
Volumen102
N.º20
DOI
EstadoPublicada - oct 2011

Áreas temáticas de ASJC Scopus

  • Bioingeniería
  • Ingeniería ambiental
  • Energías renovables, sostenibilidad y medio ambiente
  • Gestión y eliminación de residuos

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