Resumen
DNA-dependent RNA polymerase from E. coli is rapidly inhibited by preincubation with pyridoxal 5′-phosphate. The enzyme is not inhibited by pyridoxamine 5′-phosphate or pyridoxine. Pyridoxal is about 10 times less effective than pyridoxal 5′-phosphate. Nucleoside triphosphates but not DNA, protect the enzyme from inhibition. Spectral data from the reaction mixture and NaBH4 reduction products indicate the formation of a Schiff base between the aldehyde group of pyridoxal 5′-phosphate and amino group of the enzyme. The results show that the inhibitor is reacting with a critical amino group presumably at the nucleoside phosphate binding site of the active center of the enzyme.
Idioma original | Inglés |
---|---|
Páginas (desde-hasta) | 1053-1059 |
Número de páginas | 7 |
Publicación | Biochemical and Biophysical Research Communications |
Volumen | 55 |
N.º | 4 |
DOI | |
Estado | Publicada - 19 dic. 1973 |
Áreas temáticas de ASJC Scopus
- Biofísica
- Bioquímica
- Biología molecular
- Biología celular