Active site-directed inhibition of E. coli DNA-dependent RNA polymerase by pyridoxal 5′-phosphate

Alejandro Venegas, Joseph Martial, Pablo Valenzuela

Resultado de la investigación: Contribución a una revistaArtículo

39 Citas (Scopus)

Resumen

DNA-dependent RNA polymerase from E. coli is rapidly inhibited by preincubation with pyridoxal 5′-phosphate. The enzyme is not inhibited by pyridoxamine 5′-phosphate or pyridoxine. Pyridoxal is about 10 times less effective than pyridoxal 5′-phosphate. Nucleoside triphosphates but not DNA, protect the enzyme from inhibition. Spectral data from the reaction mixture and NaBH4 reduction products indicate the formation of a Schiff base between the aldehyde group of pyridoxal 5′-phosphate and amino group of the enzyme. The results show that the inhibitor is reacting with a critical amino group presumably at the nucleoside phosphate binding site of the active center of the enzyme.

Idioma originalInglés
Páginas (desde-hasta)1053-1059
Número de páginas7
PublicaciónBiochemical and Biophysical Research Communications
Volumen55
N.º4
DOI
EstadoPublicada - 19 dic 1973

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

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