Activation of the template activity of isolated rat liver nuclei for DNA synthesis and its inhibition by NAD

L. Burzio, S. S. Koide

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

33 Citas (Scopus)

Resumen

The template activity of isolated rat liver nuclei for DNA synthesis assayed with E.coli DNA polymerase was found to be dependent upon the presence of Ca2+ or Mg2+ in the incubation medium. DNA was prepared from isolated nuclei subjected to conditions which activated the template and centrifuged in an alkaline sucrose gradient. The distribution profile showed that smaller fragments were formed, suggesting enhancement of endonucleolytic activity. When isolated nuclei were incubated with NAD to induce poly(adenosine diphosphate ribose) formation and were subjected to the activation conditions, the template for DNA synthesis remained unchanged. The distribution profile in an alkaline sucrose gradient of DNA prepared from these nuclei and control nuclei was identical. The present findings suggest that the template-activating system for DNA synthesis was blocked when isolated nuclei were treated with NAD invitro.

Idioma originalInglés
Páginas (desde-hasta)572-579
Número de páginas8
PublicaciónBiochemical and Biophysical Research Communications
Volumen53
N.º2
DOI
EstadoPublicada - 17 jul. 1973
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

Huella

Profundice en los temas de investigación de 'Activation of the template activity of isolated rat liver nuclei for DNA synthesis and its inhibition by NAD'. En conjunto forman una huella única.

Citar esto