Resumen
The template activity of isolated rat liver nuclei for DNA synthesis assayed with E.coli DNA polymerase was found to be dependent upon the presence of Ca2+ or Mg2+ in the incubation medium. DNA was prepared from isolated nuclei subjected to conditions which activated the template and centrifuged in an alkaline sucrose gradient. The distribution profile showed that smaller fragments were formed, suggesting enhancement of endonucleolytic activity. When isolated nuclei were incubated with NAD to induce poly(adenosine diphosphate ribose) formation and were subjected to the activation conditions, the template for DNA synthesis remained unchanged. The distribution profile in an alkaline sucrose gradient of DNA prepared from these nuclei and control nuclei was identical. The present findings suggest that the template-activating system for DNA synthesis was blocked when isolated nuclei were treated with NAD invitro.
Idioma original | Inglés |
---|---|
Páginas (desde-hasta) | 572-579 |
Número de páginas | 8 |
Publicación | Biochemical and Biophysical Research Communications |
Volumen | 53 |
N.º | 2 |
DOI | |
Estado | Publicada - 17 jul. 1973 |
Publicado de forma externa | Sí |
Áreas temáticas de ASJC Scopus
- Biofísica
- Bioquímica
- Biología molecular
- Biología celular