Acetylcholinesterase like that of skeletal muscle in smooth muscle reinnervated by a motor nerve [20]

Nibaldo C. Inestrosa, Bernardita Mendez, Joaquin V. Luco

Resultado de la investigación: Letter

33 Citas (Scopus)

Resumen

SKELETAL muscles contain several molecular forms of acetylcholinesterase (AChE) characterised by different sedimentation coefficients1-4. In rat muscle1,2 (but not human3 and chicken5 muscle) the heavy form of AChE is exclusively present at the motor endplate regions. In contrast, the heavy form of AChE has not been detected in cat 6 or rat2 smooth muscles. The endplate AChE of rat skeletal muscle is selectively detached by treatment with collagenase 1,7, an enzyme which has been shown specifically to digest the collagen-like tail of the heavy form of AChE from several sources8,9. The presence of endplate AChE is neurally regulated10, and there is evidence that this effect is partially mediated by muscle activity11 and by a trophic factor conveyed by axoplasmic transport12,13. Previous results from our laboratory have shown that reinnervation of the cat nictitating membrane smooth muscle by a motor nerve results in an increase of AChE activity14. We report here that collagenase treatment detaches active AChE from cat smooth muscle reinnervated by a cholinergic motor nerve, and that a new AChE form with a high sedimentation coefficient is induced in these muscles. Our results suggest that the presence of the motor nerve determines, in reinnervated smooth muscle, the appearance of an AChE similar to that found in skeletal muscle.

Idioma originalEnglish
Páginas (desde-hasta)504-506
Número de páginas3
PublicaciónNature
Volumen280
N.º5722
DOI
EstadoPublished - 1979

Huella dactilar

Acetylcholinesterase
Smooth Muscle
Skeletal Muscle
Muscles
Cats
Nictitating Membrane
Motor Endplate
Collagenases
Cholinergic Agents
Tail
Collagen
Enzymes

ASJC Scopus subject areas

  • Medicine(all)
  • General

Citar esto

Inestrosa, Nibaldo C. ; Mendez, Bernardita ; Luco, Joaquin V. / Acetylcholinesterase like that of skeletal muscle in smooth muscle reinnervated by a motor nerve [20]. En: Nature. 1979 ; Vol. 280, N.º 5722. pp. 504-506.
@article{d5a1be7df65844668a0e66025c189e20,
title = "Acetylcholinesterase like that of skeletal muscle in smooth muscle reinnervated by a motor nerve [20]",
abstract = "SKELETAL muscles contain several molecular forms of acetylcholinesterase (AChE) characterised by different sedimentation coefficients1-4. In rat muscle1,2 (but not human3 and chicken5 muscle) the heavy form of AChE is exclusively present at the motor endplate regions. In contrast, the heavy form of AChE has not been detected in cat 6 or rat2 smooth muscles. The endplate AChE of rat skeletal muscle is selectively detached by treatment with collagenase 1,7, an enzyme which has been shown specifically to digest the collagen-like tail of the heavy form of AChE from several sources8,9. The presence of endplate AChE is neurally regulated10, and there is evidence that this effect is partially mediated by muscle activity11 and by a trophic factor conveyed by axoplasmic transport12,13. Previous results from our laboratory have shown that reinnervation of the cat nictitating membrane smooth muscle by a motor nerve results in an increase of AChE activity14. We report here that collagenase treatment detaches active AChE from cat smooth muscle reinnervated by a cholinergic motor nerve, and that a new AChE form with a high sedimentation coefficient is induced in these muscles. Our results suggest that the presence of the motor nerve determines, in reinnervated smooth muscle, the appearance of an AChE similar to that found in skeletal muscle.",
author = "Inestrosa, {Nibaldo C.} and Bernardita Mendez and Luco, {Joaquin V.}",
year = "1979",
doi = "10.1038/280504a0",
language = "English",
volume = "280",
pages = "504--506",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5722",

}

Acetylcholinesterase like that of skeletal muscle in smooth muscle reinnervated by a motor nerve [20]. / Inestrosa, Nibaldo C.; Mendez, Bernardita; Luco, Joaquin V.

En: Nature, Vol. 280, N.º 5722, 1979, p. 504-506.

Resultado de la investigación: Letter

TY - JOUR

T1 - Acetylcholinesterase like that of skeletal muscle in smooth muscle reinnervated by a motor nerve [20]

AU - Inestrosa, Nibaldo C.

AU - Mendez, Bernardita

AU - Luco, Joaquin V.

PY - 1979

Y1 - 1979

N2 - SKELETAL muscles contain several molecular forms of acetylcholinesterase (AChE) characterised by different sedimentation coefficients1-4. In rat muscle1,2 (but not human3 and chicken5 muscle) the heavy form of AChE is exclusively present at the motor endplate regions. In contrast, the heavy form of AChE has not been detected in cat 6 or rat2 smooth muscles. The endplate AChE of rat skeletal muscle is selectively detached by treatment with collagenase 1,7, an enzyme which has been shown specifically to digest the collagen-like tail of the heavy form of AChE from several sources8,9. The presence of endplate AChE is neurally regulated10, and there is evidence that this effect is partially mediated by muscle activity11 and by a trophic factor conveyed by axoplasmic transport12,13. Previous results from our laboratory have shown that reinnervation of the cat nictitating membrane smooth muscle by a motor nerve results in an increase of AChE activity14. We report here that collagenase treatment detaches active AChE from cat smooth muscle reinnervated by a cholinergic motor nerve, and that a new AChE form with a high sedimentation coefficient is induced in these muscles. Our results suggest that the presence of the motor nerve determines, in reinnervated smooth muscle, the appearance of an AChE similar to that found in skeletal muscle.

AB - SKELETAL muscles contain several molecular forms of acetylcholinesterase (AChE) characterised by different sedimentation coefficients1-4. In rat muscle1,2 (but not human3 and chicken5 muscle) the heavy form of AChE is exclusively present at the motor endplate regions. In contrast, the heavy form of AChE has not been detected in cat 6 or rat2 smooth muscles. The endplate AChE of rat skeletal muscle is selectively detached by treatment with collagenase 1,7, an enzyme which has been shown specifically to digest the collagen-like tail of the heavy form of AChE from several sources8,9. The presence of endplate AChE is neurally regulated10, and there is evidence that this effect is partially mediated by muscle activity11 and by a trophic factor conveyed by axoplasmic transport12,13. Previous results from our laboratory have shown that reinnervation of the cat nictitating membrane smooth muscle by a motor nerve results in an increase of AChE activity14. We report here that collagenase treatment detaches active AChE from cat smooth muscle reinnervated by a cholinergic motor nerve, and that a new AChE form with a high sedimentation coefficient is induced in these muscles. Our results suggest that the presence of the motor nerve determines, in reinnervated smooth muscle, the appearance of an AChE similar to that found in skeletal muscle.

UR - http://www.scopus.com/inward/record.url?scp=0018360694&partnerID=8YFLogxK

U2 - 10.1038/280504a0

DO - 10.1038/280504a0

M3 - Letter

C2 - 460431

AN - SCOPUS:0018360694

VL - 280

SP - 504

EP - 506

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5722

ER -