A structural motif of acetylcholinesterase that promotes amyloid β-peptide fibril formation

G. V. De Ferrari, M. A. Canales, I. Shin, L. M. Weiner, I. Silman, N. C. Inestrosa

Resultado de la investigación: Article

293 Citas (Scopus)

Resumen

Acetylcholinesterase (AChE) has been found to be associated with the core of senile plaques. We have shown that AChE interacts with the amyloid β-peptide (Aβ) and promotes amyloid fibril formation by a hydrophobic environment close to the peripheral anionic binding site (PAS) of the enzyme. Here we present evidence for the structural motif of AChE involved in this interaction. First, we modeled the docking of Aβ onto the structure of Torpedo californica AChE, and identified four potential sites for AChE-Aβ complex formation. One of these, Site L spans a major hydrophobic sequence exposed on the surface of AChE, which had been previously shown to interact with liposomes [Shin et al. (1996) Protein Sci. 5, 42-51]. Second, we examined several AChE-derived peptides and found that a synthetic 35-residue peptide corresponding to the above hydrophobic sequence was able to promote amyloid formation. We also studied the ability to promote amyloid formation of two synthetic 24-residue peptides derived from the sequence of a Ω-loop, which has been suggested as anAChE - Aβ interacting motif. Kinetic analyses indicate that only the 35-residue hydrophobic peptide mimics the effect of intact AChE on amyloid formation. Moreover, RP-HPLC analysis revealed that the 35-residue peptide was incorporated into the growing Aβ-fibrils. Finally, fluorescence binding studies showed that this peptide binds Aβ with a Kd = 184 μM, independent of salt concentration, indicating that the interaction is primarily hydrophobic. Our results indicate that the homologous human AChE motif is capable of accelerating Aβ fibrillogenesis.

Idioma originalEnglish
Páginas (desde-hasta)10447-10457
Número de páginas11
PublicaciónBiochemistry
Volumen40
N.º35
DOI
EstadoPublished - 4 sep 2001

Huella dactilar

Acetylcholinesterase
Amyloid
Peptides
Torpedo
Amyloid Plaques
Liposomes
Salts
Fluorescence
Binding Sites
High Pressure Liquid Chromatography
Kinetics
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Citar esto

De Ferrari, G. V., Canales, M. A., Shin, I., Weiner, L. M., Silman, I., & Inestrosa, N. C. (2001). A structural motif of acetylcholinesterase that promotes amyloid β-peptide fibril formation. Biochemistry, 40(35), 10447-10457. https://doi.org/10.1021/bi0101392
De Ferrari, G. V. ; Canales, M. A. ; Shin, I. ; Weiner, L. M. ; Silman, I. ; Inestrosa, N. C. / A structural motif of acetylcholinesterase that promotes amyloid β-peptide fibril formation. En: Biochemistry. 2001 ; Vol. 40, N.º 35. pp. 10447-10457.
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abstract = "Acetylcholinesterase (AChE) has been found to be associated with the core of senile plaques. We have shown that AChE interacts with the amyloid β-peptide (Aβ) and promotes amyloid fibril formation by a hydrophobic environment close to the peripheral anionic binding site (PAS) of the enzyme. Here we present evidence for the structural motif of AChE involved in this interaction. First, we modeled the docking of Aβ onto the structure of Torpedo californica AChE, and identified four potential sites for AChE-Aβ complex formation. One of these, Site L spans a major hydrophobic sequence exposed on the surface of AChE, which had been previously shown to interact with liposomes [Shin et al. (1996) Protein Sci. 5, 42-51]. Second, we examined several AChE-derived peptides and found that a synthetic 35-residue peptide corresponding to the above hydrophobic sequence was able to promote amyloid formation. We also studied the ability to promote amyloid formation of two synthetic 24-residue peptides derived from the sequence of a Ω-loop, which has been suggested as anAChE - Aβ interacting motif. Kinetic analyses indicate that only the 35-residue hydrophobic peptide mimics the effect of intact AChE on amyloid formation. Moreover, RP-HPLC analysis revealed that the 35-residue peptide was incorporated into the growing Aβ-fibrils. Finally, fluorescence binding studies showed that this peptide binds Aβ with a Kd = 184 μM, independent of salt concentration, indicating that the interaction is primarily hydrophobic. Our results indicate that the homologous human AChE motif is capable of accelerating Aβ fibrillogenesis.",
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De Ferrari, GV, Canales, MA, Shin, I, Weiner, LM, Silman, I & Inestrosa, NC 2001, 'A structural motif of acetylcholinesterase that promotes amyloid β-peptide fibril formation', Biochemistry, vol. 40, n.º 35, pp. 10447-10457. https://doi.org/10.1021/bi0101392

A structural motif of acetylcholinesterase that promotes amyloid β-peptide fibril formation. / De Ferrari, G. V.; Canales, M. A.; Shin, I.; Weiner, L. M.; Silman, I.; Inestrosa, N. C.

En: Biochemistry, Vol. 40, N.º 35, 04.09.2001, p. 10447-10457.

Resultado de la investigación: Article

TY - JOUR

T1 - A structural motif of acetylcholinesterase that promotes amyloid β-peptide fibril formation

AU - De Ferrari, G. V.

AU - Canales, M. A.

AU - Shin, I.

AU - Weiner, L. M.

AU - Silman, I.

AU - Inestrosa, N. C.

PY - 2001/9/4

Y1 - 2001/9/4

N2 - Acetylcholinesterase (AChE) has been found to be associated with the core of senile plaques. We have shown that AChE interacts with the amyloid β-peptide (Aβ) and promotes amyloid fibril formation by a hydrophobic environment close to the peripheral anionic binding site (PAS) of the enzyme. Here we present evidence for the structural motif of AChE involved in this interaction. First, we modeled the docking of Aβ onto the structure of Torpedo californica AChE, and identified four potential sites for AChE-Aβ complex formation. One of these, Site L spans a major hydrophobic sequence exposed on the surface of AChE, which had been previously shown to interact with liposomes [Shin et al. (1996) Protein Sci. 5, 42-51]. Second, we examined several AChE-derived peptides and found that a synthetic 35-residue peptide corresponding to the above hydrophobic sequence was able to promote amyloid formation. We also studied the ability to promote amyloid formation of two synthetic 24-residue peptides derived from the sequence of a Ω-loop, which has been suggested as anAChE - Aβ interacting motif. Kinetic analyses indicate that only the 35-residue hydrophobic peptide mimics the effect of intact AChE on amyloid formation. Moreover, RP-HPLC analysis revealed that the 35-residue peptide was incorporated into the growing Aβ-fibrils. Finally, fluorescence binding studies showed that this peptide binds Aβ with a Kd = 184 μM, independent of salt concentration, indicating that the interaction is primarily hydrophobic. Our results indicate that the homologous human AChE motif is capable of accelerating Aβ fibrillogenesis.

AB - Acetylcholinesterase (AChE) has been found to be associated with the core of senile plaques. We have shown that AChE interacts with the amyloid β-peptide (Aβ) and promotes amyloid fibril formation by a hydrophobic environment close to the peripheral anionic binding site (PAS) of the enzyme. Here we present evidence for the structural motif of AChE involved in this interaction. First, we modeled the docking of Aβ onto the structure of Torpedo californica AChE, and identified four potential sites for AChE-Aβ complex formation. One of these, Site L spans a major hydrophobic sequence exposed on the surface of AChE, which had been previously shown to interact with liposomes [Shin et al. (1996) Protein Sci. 5, 42-51]. Second, we examined several AChE-derived peptides and found that a synthetic 35-residue peptide corresponding to the above hydrophobic sequence was able to promote amyloid formation. We also studied the ability to promote amyloid formation of two synthetic 24-residue peptides derived from the sequence of a Ω-loop, which has been suggested as anAChE - Aβ interacting motif. Kinetic analyses indicate that only the 35-residue hydrophobic peptide mimics the effect of intact AChE on amyloid formation. Moreover, RP-HPLC analysis revealed that the 35-residue peptide was incorporated into the growing Aβ-fibrils. Finally, fluorescence binding studies showed that this peptide binds Aβ with a Kd = 184 μM, independent of salt concentration, indicating that the interaction is primarily hydrophobic. Our results indicate that the homologous human AChE motif is capable of accelerating Aβ fibrillogenesis.

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