A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli

C. Henes, M. Krauskopf, J. Ofengand

Resultado de la investigación: Article

21 Citas (Scopus)

Resumen

Phenoxyacetylation of methionyl transfer ribonucleic acid of Escherichia coli and fractionation on BD-cellulose at 4° was shown to be a rapid and convenient method for obtaining highly purified formylmethionine transfer ribonucleic acid and methionine ribonucleic acid well separated from each other. In a single run 80% pure formylmethionine transfer ribonucleic acid and 35 % pure methionine ribonucleic acid were obtained. If the chromatography was performed at 22°, ribonucleic acids specific for methionine could also be obtained highly purified but the isoaccepting species were not separated from each other. Rechromatography of material from the 4° column resulted in further purification of both ribonucleic acids, approaching 100% for formylmethionine transfer ribonucleic acid and about 70% for methionine ribonucleic acid. The order of elution of the phenoxyacetylmethionyl derivatives of formylmethionine transfer ribonucleic acid and methionine ribonucleic acid was inverted with respect to the two uncharged or aminoacylated ribonucleic acid species. It is suggested that N acylation of the methionine residue has induced a differential conformational change in the two ribonucleic acid structures which is detected by altered affinity for the BD-cellulose.

Idioma originalEnglish
Páginas (desde-hasta)3024-3028
Número de páginas5
PublicaciónBiochemistry
Volumen8
N.º7
EstadoPublished - 1969

Huella dactilar

Transfer RNA
Methionine
Escherichia coli
RNA
Acylation
Distillation columns
Fractionation
Chromatography
Purification
Derivatives

ASJC Scopus subject areas

  • Biochemistry

Citar esto

@article{9d98d9debd67456badfc33c8433a0b35,
title = "A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli",
abstract = "Phenoxyacetylation of methionyl transfer ribonucleic acid of Escherichia coli and fractionation on BD-cellulose at 4° was shown to be a rapid and convenient method for obtaining highly purified formylmethionine transfer ribonucleic acid and methionine ribonucleic acid well separated from each other. In a single run 80{\%} pure formylmethionine transfer ribonucleic acid and 35 {\%} pure methionine ribonucleic acid were obtained. If the chromatography was performed at 22°, ribonucleic acids specific for methionine could also be obtained highly purified but the isoaccepting species were not separated from each other. Rechromatography of material from the 4° column resulted in further purification of both ribonucleic acids, approaching 100{\%} for formylmethionine transfer ribonucleic acid and about 70{\%} for methionine ribonucleic acid. The order of elution of the phenoxyacetylmethionyl derivatives of formylmethionine transfer ribonucleic acid and methionine ribonucleic acid was inverted with respect to the two uncharged or aminoacylated ribonucleic acid species. It is suggested that N acylation of the methionine residue has induced a differential conformational change in the two ribonucleic acid structures which is detected by altered affinity for the BD-cellulose.",
author = "C. Henes and M. Krauskopf and J. Ofengand",
year = "1969",
language = "English",
volume = "8",
pages = "3024--3028",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "7",

}

A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli. / Henes, C.; Krauskopf, M.; Ofengand, J.

En: Biochemistry, Vol. 8, N.º 7, 1969, p. 3024-3028.

Resultado de la investigación: Article

TY - JOUR

T1 - A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli

AU - Henes, C.

AU - Krauskopf, M.

AU - Ofengand, J.

PY - 1969

Y1 - 1969

N2 - Phenoxyacetylation of methionyl transfer ribonucleic acid of Escherichia coli and fractionation on BD-cellulose at 4° was shown to be a rapid and convenient method for obtaining highly purified formylmethionine transfer ribonucleic acid and methionine ribonucleic acid well separated from each other. In a single run 80% pure formylmethionine transfer ribonucleic acid and 35 % pure methionine ribonucleic acid were obtained. If the chromatography was performed at 22°, ribonucleic acids specific for methionine could also be obtained highly purified but the isoaccepting species were not separated from each other. Rechromatography of material from the 4° column resulted in further purification of both ribonucleic acids, approaching 100% for formylmethionine transfer ribonucleic acid and about 70% for methionine ribonucleic acid. The order of elution of the phenoxyacetylmethionyl derivatives of formylmethionine transfer ribonucleic acid and methionine ribonucleic acid was inverted with respect to the two uncharged or aminoacylated ribonucleic acid species. It is suggested that N acylation of the methionine residue has induced a differential conformational change in the two ribonucleic acid structures which is detected by altered affinity for the BD-cellulose.

AB - Phenoxyacetylation of methionyl transfer ribonucleic acid of Escherichia coli and fractionation on BD-cellulose at 4° was shown to be a rapid and convenient method for obtaining highly purified formylmethionine transfer ribonucleic acid and methionine ribonucleic acid well separated from each other. In a single run 80% pure formylmethionine transfer ribonucleic acid and 35 % pure methionine ribonucleic acid were obtained. If the chromatography was performed at 22°, ribonucleic acids specific for methionine could also be obtained highly purified but the isoaccepting species were not separated from each other. Rechromatography of material from the 4° column resulted in further purification of both ribonucleic acids, approaching 100% for formylmethionine transfer ribonucleic acid and about 70% for methionine ribonucleic acid. The order of elution of the phenoxyacetylmethionyl derivatives of formylmethionine transfer ribonucleic acid and methionine ribonucleic acid was inverted with respect to the two uncharged or aminoacylated ribonucleic acid species. It is suggested that N acylation of the methionine residue has induced a differential conformational change in the two ribonucleic acid structures which is detected by altered affinity for the BD-cellulose.

UR - http://www.scopus.com/inward/record.url?scp=0014545273&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 3024

EP - 3028

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 7

ER -