A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael Quigley

Resultado de la investigación: Contribución a una revistaArtículo

1985 Citas (Scopus)

Resumen

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15-40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and can be resuspended in buffer and immediately restricted. A 5-ml bacterial culture yields enough plasmids for many restriction enzyme digestions, but a single colony on a petri dish or a 0.5-ml miniculture will suffice for a few experiments. In addition, the procedure can be readily adapted for the preparation of plasmids from liter cultures with quantitative yields.

Idioma originalInglés
Páginas (desde-hasta)193-197
Número de páginas5
PublicaciónAnalytical Biochemistry
Volumen114
N.º1
DOI
EstadoPublicada - jun 1981

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

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