A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael Quigley

Resultado de la investigación: Article

1971 Citas (Scopus)

Resumen

A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15-40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and can be resuspended in buffer and immediately restricted. A 5-ml bacterial culture yields enough plasmids for many restriction enzyme digestions, but a single colony on a petri dish or a 0.5-ml miniculture will suffice for a few experiments. In addition, the procedure can be readily adapted for the preparation of plasmids from liter cultures with quantitative yields.

Idioma originalEnglish
Páginas (desde-hasta)193-197
Número de páginas5
PublicaciónAnalytical Biochemistry
Volumen114
N.º1
DOI
EstadoPublished - 1981

Huella dactilar

Boiling liquids
Plasmids
Restriction Mapping
2-Propanol
Centrifugation
Enzymes
Debris
Digestion
Bacteria
Buffers
DNA
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Citar esto

Holmes, David S. ; Quigley, Michael. / A rapid boiling method for the preparation of bacterial plasmids. En: Analytical Biochemistry. 1981 ; Vol. 114, N.º 1. pp. 193-197.
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A rapid boiling method for the preparation of bacterial plasmids. / Holmes, David S.; Quigley, Michael.

En: Analytical Biochemistry, Vol. 114, N.º 1, 1981, p. 193-197.

Resultado de la investigación: Article

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