A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum

Gloria León, Nancy Maulén, Jaime Figueroa, Julieta Villanueva, Claudia Rodríguez, María Inés Vera, Manuel Krauskopf

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

28 Citas (Scopus)

Resumen

By means of a one-step one-tube extraction from less than 1 mg of tissue it is possible to identify, via the polymerase chain reaction, Renibacterium salmoninarum in salmon with bacterial kidney disease. A 149-bp DNA sequence unique to R salmoninarum was specifically amplified and its nature confirmed by Southern hybridization using a non-isotopically labelled probe. The sensitivity of the approach allowed the detection of 22 R. salmoninarum cells. The procedure was successfully applied in the identification of the causative agent of bacterial kidney disease in kidney tissue from infected fishes.

Idioma originalInglés
Páginas (desde-hasta)131-136
Número de páginas6
PublicaciónFEMS Microbiology Letters
Volumen115
N.º2-3
DOI
EstadoPublicada - 15 ene 1994

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular
  • Genética

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