A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II Is regulated by B3 domain transcription factors during seed development in arabidopsis

Hannetz Roschzttardtz, Ignacia Fuentes, Marcos Vásquez, Claudia Corvalán, Gabriel León, Isabel Gómez, Alejandro Araya, Loreto Holuigue, Jesús Vicente-Carbajosa, Xavier Jordana

Resultado de la investigación: Article

29 Citas (Scopus)

Resumen

Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.

Idioma originalEnglish
Páginas (desde-hasta)84-95
Número de páginas12
PublicaciónPlant Physiology
Volumen150
N.º1
DOI
EstadoPublished - 1 may 2009

Huella dactilar

Succinic Acid
succinic acid
seed development
Sulfur
Arabidopsis
Seeds
sulfur
Transcription Factors
Iron
transcription factors
iron
Abscisic Acid
Embryonic Structures
Genes
genes
succinate dehydrogenase (quinone)
promoter regions
abscisic acid
Germination
seed maturation

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Citar esto

Roschzttardtz, Hannetz ; Fuentes, Ignacia ; Vásquez, Marcos ; Corvalán, Claudia ; León, Gabriel ; Gómez, Isabel ; Araya, Alejandro ; Holuigue, Loreto ; Vicente-Carbajosa, Jesús ; Jordana, Xavier. / A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II Is regulated by B3 domain transcription factors during seed development in arabidopsis. En: Plant Physiology. 2009 ; Vol. 150, N.º 1. pp. 84-95.
@article{6db1990d7cba4c6e8114dff7ce960cd6,
title = "A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II Is regulated by B3 domain transcription factors during seed development in arabidopsis",
abstract = "Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.",
author = "Hannetz Roschzttardtz and Ignacia Fuentes and Marcos V{\'a}squez and Claudia Corval{\'a}n and Gabriel Le{\'o}n and Isabel G{\'o}mez and Alejandro Araya and Loreto Holuigue and Jes{\'u}s Vicente-Carbajosa and Xavier Jordana",
year = "2009",
month = "5",
day = "1",
doi = "10.1104/pp.109.136531",
language = "English",
volume = "150",
pages = "84--95",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "1",

}

Roschzttardtz, H, Fuentes, I, Vásquez, M, Corvalán, C, León, G, Gómez, I, Araya, A, Holuigue, L, Vicente-Carbajosa, J & Jordana, X 2009, 'A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II Is regulated by B3 domain transcription factors during seed development in arabidopsis', Plant Physiology, vol. 150, n.º 1, pp. 84-95. https://doi.org/10.1104/pp.109.136531

A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II Is regulated by B3 domain transcription factors during seed development in arabidopsis. / Roschzttardtz, Hannetz; Fuentes, Ignacia; Vásquez, Marcos; Corvalán, Claudia; León, Gabriel; Gómez, Isabel; Araya, Alejandro; Holuigue, Loreto; Vicente-Carbajosa, Jesús; Jordana, Xavier.

En: Plant Physiology, Vol. 150, N.º 1, 01.05.2009, p. 84-95.

Resultado de la investigación: Article

TY - JOUR

T1 - A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II Is regulated by B3 domain transcription factors during seed development in arabidopsis

AU - Roschzttardtz, Hannetz

AU - Fuentes, Ignacia

AU - Vásquez, Marcos

AU - Corvalán, Claudia

AU - León, Gabriel

AU - Gómez, Isabel

AU - Araya, Alejandro

AU - Holuigue, Loreto

AU - Vicente-Carbajosa, Jesús

AU - Jordana, Xavier

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.

AB - Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.

UR - http://www.scopus.com/inward/record.url?scp=66149146613&partnerID=8YFLogxK

U2 - 10.1104/pp.109.136531

DO - 10.1104/pp.109.136531

M3 - Article

C2 - 19261733

AN - SCOPUS:66149146613

VL - 150

SP - 84

EP - 95

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 1

ER -