A novel monoclonal antibody against the c-terminus of β-tubulin recognizes endocytic organelles in trypanosoma cruzi

Alberto Cornejo, Claudio Rogério De Oliveira, Martin Würtele, Janete Chung, Kai Hilpert, Sergio Schenkman

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

Microtubule cytoskeleton is a dynamic structure involved in the maintenance of eukaryote cell shape, motion of cilia and flagellum, and intracellular movement of vesicles and organelles. Many antibodies against tubulins have been described, most of them against the C-terminal portion, which is exposed at the outside of the microtubules. By generating a novel set of monoclonal antibodies against the cytoskeleton of Trypanosoma cruzi, a flagellate protozoan that causes Chagas' disease, we selected a clone (mAb 3G4) that recognizes β-tubulin. The epitope for mAb 3G4 was mapped by pepscan to a highly conserved sequence motif found between α-helices 11 and 12 of the C-terminus of -tubulin in eukaryotes. It labels vesicular structures in both T. cruzi and mammalian cells, colocalizing respectively with a major cysteine protease (Cruzipain) and lysosome associated protein (LAMP2) respectively, but it does not label regular microtubules on these cellular models. We propose that the epitope recognized by mAb 3G4 is exposed only in a form of tubulin associated with endosomes.

Idioma originalEnglish
Páginas (desde-hasta)636-643
Número de páginas8
PublicaciónProtein and Peptide Letters
Volumen19
N.º6
DOI
EstadoPublished - 1 ene 2012

Huella dactilar

Trypanosoma cruzi
Tubulin
Organelles
Monoclonal Antibodies
Microtubules
Eukaryota
Cytoskeleton
Lysosomal-Associated Membrane Protein 2
Labels
Epitopes
Cysteine Proteases
Flagella
Chagas Disease
Cell Shape
Conserved Sequence
Cilia
Endosomes
Lysosomes
Clone Cells
Cells

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Citar esto

Cornejo, Alberto ; De Oliveira, Claudio Rogério ; Würtele, Martin ; Chung, Janete ; Hilpert, Kai ; Schenkman, Sergio. / A novel monoclonal antibody against the c-terminus of β-tubulin recognizes endocytic organelles in trypanosoma cruzi. En: Protein and Peptide Letters. 2012 ; Vol. 19, N.º 6. pp. 636-643.
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abstract = "Microtubule cytoskeleton is a dynamic structure involved in the maintenance of eukaryote cell shape, motion of cilia and flagellum, and intracellular movement of vesicles and organelles. Many antibodies against tubulins have been described, most of them against the C-terminal portion, which is exposed at the outside of the microtubules. By generating a novel set of monoclonal antibodies against the cytoskeleton of Trypanosoma cruzi, a flagellate protozoan that causes Chagas' disease, we selected a clone (mAb 3G4) that recognizes β-tubulin. The epitope for mAb 3G4 was mapped by pepscan to a highly conserved sequence motif found between α-helices 11 and 12 of the C-terminus of -tubulin in eukaryotes. It labels vesicular structures in both T. cruzi and mammalian cells, colocalizing respectively with a major cysteine protease (Cruzipain) and lysosome associated protein (LAMP2) respectively, but it does not label regular microtubules on these cellular models. We propose that the epitope recognized by mAb 3G4 is exposed only in a form of tubulin associated with endosomes.",
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A novel monoclonal antibody against the c-terminus of β-tubulin recognizes endocytic organelles in trypanosoma cruzi. / Cornejo, Alberto; De Oliveira, Claudio Rogério; Würtele, Martin; Chung, Janete; Hilpert, Kai; Schenkman, Sergio.

En: Protein and Peptide Letters, Vol. 19, N.º 6, 01.01.2012, p. 636-643.

Resultado de la investigación: Article

TY - JOUR

T1 - A novel monoclonal antibody against the c-terminus of β-tubulin recognizes endocytic organelles in trypanosoma cruzi

AU - Cornejo, Alberto

AU - De Oliveira, Claudio Rogério

AU - Würtele, Martin

AU - Chung, Janete

AU - Hilpert, Kai

AU - Schenkman, Sergio

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Microtubule cytoskeleton is a dynamic structure involved in the maintenance of eukaryote cell shape, motion of cilia and flagellum, and intracellular movement of vesicles and organelles. Many antibodies against tubulins have been described, most of them against the C-terminal portion, which is exposed at the outside of the microtubules. By generating a novel set of monoclonal antibodies against the cytoskeleton of Trypanosoma cruzi, a flagellate protozoan that causes Chagas' disease, we selected a clone (mAb 3G4) that recognizes β-tubulin. The epitope for mAb 3G4 was mapped by pepscan to a highly conserved sequence motif found between α-helices 11 and 12 of the C-terminus of -tubulin in eukaryotes. It labels vesicular structures in both T. cruzi and mammalian cells, colocalizing respectively with a major cysteine protease (Cruzipain) and lysosome associated protein (LAMP2) respectively, but it does not label regular microtubules on these cellular models. We propose that the epitope recognized by mAb 3G4 is exposed only in a form of tubulin associated with endosomes.

AB - Microtubule cytoskeleton is a dynamic structure involved in the maintenance of eukaryote cell shape, motion of cilia and flagellum, and intracellular movement of vesicles and organelles. Many antibodies against tubulins have been described, most of them against the C-terminal portion, which is exposed at the outside of the microtubules. By generating a novel set of monoclonal antibodies against the cytoskeleton of Trypanosoma cruzi, a flagellate protozoan that causes Chagas' disease, we selected a clone (mAb 3G4) that recognizes β-tubulin. The epitope for mAb 3G4 was mapped by pepscan to a highly conserved sequence motif found between α-helices 11 and 12 of the C-terminus of -tubulin in eukaryotes. It labels vesicular structures in both T. cruzi and mammalian cells, colocalizing respectively with a major cysteine protease (Cruzipain) and lysosome associated protein (LAMP2) respectively, but it does not label regular microtubules on these cellular models. We propose that the epitope recognized by mAb 3G4 is exposed only in a form of tubulin associated with endosomes.

KW - Epitope

KW - Lysosome,endosome

KW - Monoclonal antibody

KW - Trypanosoma cruzi

KW - Tubulin

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