A novel chimeric mitochondrial RNA localized in the nucleus of mouse sperm

J. Villegas, A. M. Zarraga, I. Muller, L. Montecinos, E. Werner, M. Brito, A. M. Meneses, L. O. Burzio

Resultado de la investigación: Article

20 Citas (Scopus)

Resumen

Six identical cDNA clones corresponding to an RNA of 1685 nucleotides that is enriched in mouse sperm compared with testis were isolated from a mouse testis cDNA library. The sequence of these clones corresponds to the 16S mitochondrial RNA plus an inverted repeat of 120 bp covalently joined to the 5' end of the RNA. By RT-PCR, it was demonstrated that this transcript, referred to as chimeric RNA, was present in mouse sperm, testis, liver, kidney, brain, and spleen. The absence of an equivalent sequence in mitochondrial DNA or as a mitochondrial pseudogene in total DNA extracted from sperm, testis, and somatic tissues suggests that the chimeric RNA is a post-transcriptional product, maybe resulting from a trans splicing reaction. The chimeric RNA was found by RT-PCR in total RNA extracted from purified sperm heads. This result was confirmed by in situ hybridization, which showed clear staining of the sperm nucleus with probes corresponding to sequences of the mitochondrial 16S RNA and the inverted repeat.

Idioma originalEnglish
Páginas (desde-hasta)579-588
Número de páginas10
PublicaciónDNA and Cell Biology
Volumen19
N.º9
DOI
EstadoPublished - 2000

Huella dactilar

Spermatozoa
RNA
Testis
Clone Cells
Trans-Splicing
Sperm Head
Polymerase Chain Reaction
Pseudogenes
Mitochondrial DNA
Gene Library
In Situ Hybridization
mitochondrial RNA
Spleen
Nucleotides
Complementary DNA
Staining and Labeling
Kidney
Liver
DNA
Brain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology
  • Medicine(all)

Citar esto

Villegas, J., Zarraga, A. M., Muller, I., Montecinos, L., Werner, E., Brito, M., ... Burzio, L. O. (2000). A novel chimeric mitochondrial RNA localized in the nucleus of mouse sperm. DNA and Cell Biology, 19(9), 579-588. https://doi.org/10.1089/104454900439809
Villegas, J. ; Zarraga, A. M. ; Muller, I. ; Montecinos, L. ; Werner, E. ; Brito, M. ; Meneses, A. M. ; Burzio, L. O. / A novel chimeric mitochondrial RNA localized in the nucleus of mouse sperm. En: DNA and Cell Biology. 2000 ; Vol. 19, N.º 9. pp. 579-588.
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abstract = "Six identical cDNA clones corresponding to an RNA of 1685 nucleotides that is enriched in mouse sperm compared with testis were isolated from a mouse testis cDNA library. The sequence of these clones corresponds to the 16S mitochondrial RNA plus an inverted repeat of 120 bp covalently joined to the 5' end of the RNA. By RT-PCR, it was demonstrated that this transcript, referred to as chimeric RNA, was present in mouse sperm, testis, liver, kidney, brain, and spleen. The absence of an equivalent sequence in mitochondrial DNA or as a mitochondrial pseudogene in total DNA extracted from sperm, testis, and somatic tissues suggests that the chimeric RNA is a post-transcriptional product, maybe resulting from a trans splicing reaction. The chimeric RNA was found by RT-PCR in total RNA extracted from purified sperm heads. This result was confirmed by in situ hybridization, which showed clear staining of the sperm nucleus with probes corresponding to sequences of the mitochondrial 16S RNA and the inverted repeat.",
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Villegas, J, Zarraga, AM, Muller, I, Montecinos, L, Werner, E, Brito, M, Meneses, AM & Burzio, LO 2000, 'A novel chimeric mitochondrial RNA localized in the nucleus of mouse sperm', DNA and Cell Biology, vol. 19, n.º 9, pp. 579-588. https://doi.org/10.1089/104454900439809

A novel chimeric mitochondrial RNA localized in the nucleus of mouse sperm. / Villegas, J.; Zarraga, A. M.; Muller, I.; Montecinos, L.; Werner, E.; Brito, M.; Meneses, A. M.; Burzio, L. O.

En: DNA and Cell Biology, Vol. 19, N.º 9, 2000, p. 579-588.

Resultado de la investigación: Article

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AU - Villegas, J.

AU - Zarraga, A. M.

AU - Muller, I.

AU - Montecinos, L.

AU - Werner, E.

AU - Brito, M.

AU - Meneses, A. M.

AU - Burzio, L. O.

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