A Native Ternary Complex Trapped in a Crystal Reveals the Catalytic Mechanism of a Retaining Glycosyltransferase

David Albesa-Jové, Fernanda Mendoza, Ane Rodrigo-Unzueta, Fernando Gomollõn-Bel, Javier O. Cifuente, Saioa Urresti, Natalia Comino, Hansel Gõmez, Javier Romero-García, José M. Lluch, Enea Sancho-Vaello, Xevi Biarnés, Antoni Planas, Pedro Merino, Laura Masgrau, Marcelo E. Guerin

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

24 Citas (Scopus)

Resumen

Glycosyltransferases (GTs) comprise a prominent family of enzymes that play critical roles in a variety of cellular processes, including cell signaling, cell development, and host-pathogen interactions. Glycosyl transfer can proceed with either inversion or retention of the anomeric configuration with respect to the reaction substrates and products. The elucidation of the catalytic mechanism of retaining GTs remains a major challenge. A native ternary complex of a GT in a productive mode for catalysis is reported, that of the retaining glucosyl-3-phosphoglycerate synthase GpgS from M. tuberculosis in the presence of the sugar donor UDP-Glc, the acceptor substrate phosphoglycerate, and the divalent cation cofactor. Through a combination of structural, chemical, enzymatic, molecular dynamics, and quantum-mechanics/molecular-mechanics (QM/MM) calculations, the catalytic mechanism was unraveled, thereby providing a strong experimental support for a front-side substrate-assisted SNi-type reaction. Pass the sugar: The crystal structure of a native ternary complex of a glycosyltransferase, the retaining glucosyl-3-phosphoglycerate synthase GpgS, in a productive mode for catalysis was obtained. By combining structural, chemical, and enzymatic methods, as well as molecular dynamics and QM/MM calculations, the catalytic mechanism was unraveled and the results provide strong experimental support for a front-side substrate-assisted SNi-type reaction.

Idioma originalInglés
Páginas (desde-hasta)9898-9902
Número de páginas5
PublicaciónAngewandte Chemie - International Edition
Volumen54
N.º34
DOI
EstadoPublicada - 1 ago 2015
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Catálisis
  • Química (todo)

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