A Method for Isolation of Intact, Translationally Active Ribonucleic Acid

Guy Cathala, Jean Francois Savouret, Bernardita Mendez, Brian L. West, Michael Karin, Joseph A. Martial, John D. Baxter

Resultado de la investigación: Article

1190 Citas (Scopus)

Resumen

A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (<300 nucleotides) RNA species are recovered with a poor yield.

Idioma originalEnglish
Páginas (desde-hasta)329-335
Número de páginas7
PublicaciónDNA
Volumen2
N.º4
DOI
EstadoPublished - 1983

Huella dactilar

Guanidine
RNA
Tissue
Tissue culture
RNA Precursors
Yeast
Ultracentrifugation
Nucleotides
Messenger RNA
Cell Culture Techniques
Yeasts

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Citar esto

Cathala, G., Savouret, J. F., Mendez, B., West, B. L., Karin, M., Martial, J. A., & Baxter, J. D. (1983). A Method for Isolation of Intact, Translationally Active Ribonucleic Acid. DNA, 2(4), 329-335. https://doi.org/10.1089/dna.1983.2.329
Cathala, Guy ; Savouret, Jean Francois ; Mendez, Bernardita ; West, Brian L. ; Karin, Michael ; Martial, Joseph A. ; Baxter, John D. / A Method for Isolation of Intact, Translationally Active Ribonucleic Acid. En: DNA. 1983 ; Vol. 2, N.º 4. pp. 329-335.
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Cathala, G, Savouret, JF, Mendez, B, West, BL, Karin, M, Martial, JA & Baxter, JD 1983, 'A Method for Isolation of Intact, Translationally Active Ribonucleic Acid', DNA, vol. 2, n.º 4, pp. 329-335. https://doi.org/10.1089/dna.1983.2.329

A Method for Isolation of Intact, Translationally Active Ribonucleic Acid. / Cathala, Guy; Savouret, Jean Francois; Mendez, Bernardita; West, Brian L.; Karin, Michael; Martial, Joseph A.; Baxter, John D.

En: DNA, Vol. 2, N.º 4, 1983, p. 329-335.

Resultado de la investigación: Article

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AU - Cathala, Guy

AU - Savouret, Jean Francois

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AU - Karin, Michael

AU - Martial, Joseph A.

AU - Baxter, John D.

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AB - A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (<300 nucleotides) RNA species are recovered with a poor yield.

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Cathala G, Savouret JF, Mendez B, West BL, Karin M, Martial JA y otros. A Method for Isolation of Intact, Translationally Active Ribonucleic Acid. DNA. 1983;2(4):329-335. https://doi.org/10.1089/dna.1983.2.329