A Method for Isolation of Intact, Translationally Active Ribonucleic Acid

Guy Cathala, Jean Francois Savouret, Bernardita Mendez, Brian L. West, Michael Karin, Joseph A. Martial, John D. Baxter

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

1203 Citas (Scopus)

Resumen

A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (<300 nucleotides) RNA species are recovered with a poor yield.

Idioma originalInglés
Páginas (desde-hasta)329-335
Número de páginas7
PublicaciónDNA
Volumen2
N.º4
DOI
EstadoPublicada - 1983

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Genética

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