A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi

Paula I. Rodas, A. Nicole Trombert, Guido C. Mora

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1 single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.

Idioma originalEnglish
Páginas (desde-hasta)58-66
Número de páginas9
PublicaciónFEMS Microbiology Letters
Volumen321
N.º1
DOI
EstadoPublished - ago 2011

Huella dactilar

Salmonella typhi
Open Reading Frames
Gentian Violet
Galactosides
Nonsense Codon
Computational Biology
Bacteriophages
Organism Cloning
Permeability
Membrane Proteins
Proteins
Escherichia coli
Amino Acids
Membranes
Growth
Genes

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Citar esto

Rodas, Paula I. ; Trombert, A. Nicole ; Mora, Guido C. / A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi. En: FEMS Microbiology Letters. 2011 ; Vol. 321, N.º 1. pp. 58-66.
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abstract = "We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1™ single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.",
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A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi. / Rodas, Paula I.; Trombert, A. Nicole; Mora, Guido C.

En: FEMS Microbiology Letters, Vol. 321, N.º 1, 08.2011, p. 58-66.

Resultado de la investigación: Article

TY - JOUR

T1 - A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi

AU - Rodas, Paula I.

AU - Trombert, A. Nicole

AU - Mora, Guido C.

PY - 2011/8

Y1 - 2011/8

N2 - We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1™ single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.

AB - We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1™ single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.

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KW - Inner membrane

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