A functional ferric uptake regulator (Fur) protein in the fish pathogen Piscirickettsia salmonis

Oscar Almarza, Katherine Valderrama, Manuel Ayala, Cristopher Segovia, Javier Santander

Resultado de la investigación: Article

6 Citas (Scopus)

Resumen

Piscirickettsia salmonis, a Gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (SRS). The P. salmonis iron acquisition mechanisms and its molecular regulation are unknown. Iron is an essential element for bacterial pathogenesis. Typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (Fur) protein. P. salmonis fur sequence database reveals a diversity of fur genes without functional verification. Due to the fastidious nature of this bacterium, we evaluated the functionality of P. salmonis fur in the Salmonella Δfur heterologous system. Although P. salmonis fur gene strongly differed from the common Fur sequences, it restored the regulatory mechanisms of iron acquisition in Salmonella. We concluded that P. salmonis LF-89 has a conserved functional Fur protein, which reinforces the importance of iron during fish infection.

Idioma originalEnglish
Páginas (desde-hasta)49-55
Número de páginas7
PublicaciónInternational Microbiology
Volumen19
N.º1
DOI
EstadoPublished - 1 ene 2016

Huella dactilar

Piscirickettsia
Fish Proteins
Iron
Salmonella
Genes
Sepsis
Fishes
Proteins
Databases
Bacteria
Infection

ASJC Scopus subject areas

  • Microbiology
  • Medicine(all)
  • Microbiology (medical)

Citar esto

Almarza, Oscar ; Valderrama, Katherine ; Ayala, Manuel ; Segovia, Cristopher ; Santander, Javier. / A functional ferric uptake regulator (Fur) protein in the fish pathogen Piscirickettsia salmonis. En: International Microbiology. 2016 ; Vol. 19, N.º 1. pp. 49-55.
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abstract = "Piscirickettsia salmonis, a Gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (SRS). The P. salmonis iron acquisition mechanisms and its molecular regulation are unknown. Iron is an essential element for bacterial pathogenesis. Typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (Fur) protein. P. salmonis fur sequence database reveals a diversity of fur genes without functional verification. Due to the fastidious nature of this bacterium, we evaluated the functionality of P. salmonis fur in the Salmonella Δfur heterologous system. Although P. salmonis fur gene strongly differed from the common Fur sequences, it restored the regulatory mechanisms of iron acquisition in Salmonella. We concluded that P. salmonis LF-89 has a conserved functional Fur protein, which reinforces the importance of iron during fish infection.",
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A functional ferric uptake regulator (Fur) protein in the fish pathogen Piscirickettsia salmonis. / Almarza, Oscar; Valderrama, Katherine; Ayala, Manuel; Segovia, Cristopher; Santander, Javier.

En: International Microbiology, Vol. 19, N.º 1, 01.01.2016, p. 49-55.

Resultado de la investigación: Article

TY - JOUR

T1 - A functional ferric uptake regulator (Fur) protein in the fish pathogen Piscirickettsia salmonis

AU - Almarza, Oscar

AU - Valderrama, Katherine

AU - Ayala, Manuel

AU - Segovia, Cristopher

AU - Santander, Javier

PY - 2016/1/1

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N2 - Piscirickettsia salmonis, a Gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (SRS). The P. salmonis iron acquisition mechanisms and its molecular regulation are unknown. Iron is an essential element for bacterial pathogenesis. Typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (Fur) protein. P. salmonis fur sequence database reveals a diversity of fur genes without functional verification. Due to the fastidious nature of this bacterium, we evaluated the functionality of P. salmonis fur in the Salmonella Δfur heterologous system. Although P. salmonis fur gene strongly differed from the common Fur sequences, it restored the regulatory mechanisms of iron acquisition in Salmonella. We concluded that P. salmonis LF-89 has a conserved functional Fur protein, which reinforces the importance of iron during fish infection.

AB - Piscirickettsia salmonis, a Gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (SRS). The P. salmonis iron acquisition mechanisms and its molecular regulation are unknown. Iron is an essential element for bacterial pathogenesis. Typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (Fur) protein. P. salmonis fur sequence database reveals a diversity of fur genes without functional verification. Due to the fastidious nature of this bacterium, we evaluated the functionality of P. salmonis fur in the Salmonella Δfur heterologous system. Although P. salmonis fur gene strongly differed from the common Fur sequences, it restored the regulatory mechanisms of iron acquisition in Salmonella. We concluded that P. salmonis LF-89 has a conserved functional Fur protein, which reinforces the importance of iron during fish infection.

KW - Ferric uptake regulator protein (Fur)

KW - Fish pathogens

KW - Iron acquisition

KW - Piscirickettsia salmonis

KW - Transcriptional regulatory element

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