A flow cytometric procedure for the quantification of cell adhesion in complex mixtures of cells

María Rosa Bono, Lilian I. Reyes, Mario Rosemblatt

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

We present a simple non-radioactive cytometry-based assay that permits the simultaneous quantitation of cell adhesion of distinct subsets of cells contained in a mixture without any previous fractionation. The procedure is simple and highly reproducible and has the advantage of confining the quantitation of cell adhesion to live cells only. This new approach is based on counting the absolute number of cells. This is done by adding known numbers of distinguishable beads to the cell suspension and counting beads and cells in a cytometer. Quantitation of adhesion is accomplished by counting each subpopulation of cells before and after the adhesive process. To illustrate this methodology we determined adhesion of Ramos cells to monolayers of endothelial cells and its inhibition by specific antibodies. Also, we determined adhesion to endothelial cells of B lymphocytes and subsets of T lymphocytes present in a preparation of unfractionated human mononuclear cells. The results presented here demonstrate that the new assay has the required properties to be used in the quantitation of cell adhesion.

Idioma originalEnglish
Páginas (desde-hasta)27-36
Número de páginas10
PublicaciónJournal of Immunological Methods
Volumen223
N.º1
DOI
EstadoPublished - 1 feb 1999

Huella dactilar

Complex Mixtures
Cell Adhesion
Endothelial Cells
B-Lymphocyte Subsets
Adhesives
Suspensions
Cell Count
T-Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Citar esto

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abstract = "We present a simple non-radioactive cytometry-based assay that permits the simultaneous quantitation of cell adhesion of distinct subsets of cells contained in a mixture without any previous fractionation. The procedure is simple and highly reproducible and has the advantage of confining the quantitation of cell adhesion to live cells only. This new approach is based on counting the absolute number of cells. This is done by adding known numbers of distinguishable beads to the cell suspension and counting beads and cells in a cytometer. Quantitation of adhesion is accomplished by counting each subpopulation of cells before and after the adhesive process. To illustrate this methodology we determined adhesion of Ramos cells to monolayers of endothelial cells and its inhibition by specific antibodies. Also, we determined adhesion to endothelial cells of B lymphocytes and subsets of T lymphocytes present in a preparation of unfractionated human mononuclear cells. The results presented here demonstrate that the new assay has the required properties to be used in the quantitation of cell adhesion.",
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A flow cytometric procedure for the quantification of cell adhesion in complex mixtures of cells. / Bono, María Rosa; Reyes, Lilian I.; Rosemblatt, Mario.

En: Journal of Immunological Methods, Vol. 223, N.º 1, 01.02.1999, p. 27-36.

Resultado de la investigación: Article

TY - JOUR

T1 - A flow cytometric procedure for the quantification of cell adhesion in complex mixtures of cells

AU - Bono, María Rosa

AU - Reyes, Lilian I.

AU - Rosemblatt, Mario

PY - 1999/2/1

Y1 - 1999/2/1

N2 - We present a simple non-radioactive cytometry-based assay that permits the simultaneous quantitation of cell adhesion of distinct subsets of cells contained in a mixture without any previous fractionation. The procedure is simple and highly reproducible and has the advantage of confining the quantitation of cell adhesion to live cells only. This new approach is based on counting the absolute number of cells. This is done by adding known numbers of distinguishable beads to the cell suspension and counting beads and cells in a cytometer. Quantitation of adhesion is accomplished by counting each subpopulation of cells before and after the adhesive process. To illustrate this methodology we determined adhesion of Ramos cells to monolayers of endothelial cells and its inhibition by specific antibodies. Also, we determined adhesion to endothelial cells of B lymphocytes and subsets of T lymphocytes present in a preparation of unfractionated human mononuclear cells. The results presented here demonstrate that the new assay has the required properties to be used in the quantitation of cell adhesion.

AB - We present a simple non-radioactive cytometry-based assay that permits the simultaneous quantitation of cell adhesion of distinct subsets of cells contained in a mixture without any previous fractionation. The procedure is simple and highly reproducible and has the advantage of confining the quantitation of cell adhesion to live cells only. This new approach is based on counting the absolute number of cells. This is done by adding known numbers of distinguishable beads to the cell suspension and counting beads and cells in a cytometer. Quantitation of adhesion is accomplished by counting each subpopulation of cells before and after the adhesive process. To illustrate this methodology we determined adhesion of Ramos cells to monolayers of endothelial cells and its inhibition by specific antibodies. Also, we determined adhesion to endothelial cells of B lymphocytes and subsets of T lymphocytes present in a preparation of unfractionated human mononuclear cells. The results presented here demonstrate that the new assay has the required properties to be used in the quantitation of cell adhesion.

KW - Cell adhesion

KW - Flow cytometric assay

KW - Lymphocyte subpopulation adhesion analysis

KW - Mixed cell adhesion analysis

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U2 - 10.1016/S0022-1759(98)00196-3

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