A first insight into the structure and function of rhizosphere microbiota in Antarctic plants using shotgun metagenomic

Marco A. Molina-Montenegro, Gabriel I. Ballesteros, Eduardo Castro-Nallar, Claudio Meneses, Jorge Gallardo-Cerda, Cristian Torres-Díaz

Resultado de la investigación: Article

Resumen

Antarctic vascular plants such as Deschampsia antarctica (Da) could generate more suitable micro-environmental conditions for the establishment of other plants like Colobanthus quitensis (Cq). Although positive plant–plant interactions have been shown to contribute to plant performance and establishment, little is known about how microorganisms might modulate those interactions, particularly in stressful environmental conditions. Several reports have focused on the possible ecological roles of microorganisms on vascular plants, but if rhizospheric microorganisms can impact positive interactions among Antarctic plants has been seldom studied. Here, we assessed the physical–chemical characteristics of rhizospheric soils from Cq growing alone or associated with Da (Cq + Da). In addition, we compared the rhizosphere microbiomes associated with Cq, either growing alone or associated with Da (Cq + Da), using a shotgun metagenomic DNA sequencing approach and using eggNOG for comparative and functional metagenomics. Overall, there were no differences among rhizospheric soils in terms of physical–chemical characteristics. On the other hand, our results show significant differences in terms of taxonomic diversity between rhizospheric soils. Functional annotation and pathway analysis showed that microorganisms from rhizospheric soil samples also have significant differences in gene abundance associated with several functional categories related to environmental tolerance and in metabolic pathways linked to osmotic stress, among others. Overall, this study provides foundational information which will allow to explore the biological impact of the rhizobiome and its functional mechanisms and molecular pathways on plant performance and help explain the concerted strategy deployed by Cq to inhabit and cope with the harsh conditions prevailing in Antarctica.

Idioma originalEnglish
PublicaciónPolar Biology
DOI
EstadoPublished - 1 ene 2019

Huella dactilar

Metagenomics
Deschampsia antarctica
Rhizosphere
Microbiota
Firearms
rhizosphere
Soil
microorganisms
vascular plants
Blood Vessels
soil
environmental factors
plant establishment
Osmotic Pressure
osmotic stress
Metabolic Networks and Pathways
DNA Sequence Analysis
Antarctica
biochemical pathways
Colobanthus quitensis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Citar esto

Molina-Montenegro, Marco A. ; Ballesteros, Gabriel I. ; Castro-Nallar, Eduardo ; Meneses, Claudio ; Gallardo-Cerda, Jorge ; Torres-Díaz, Cristian. / A first insight into the structure and function of rhizosphere microbiota in Antarctic plants using shotgun metagenomic. En: Polar Biology. 2019.
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abstract = "Antarctic vascular plants such as Deschampsia antarctica (Da) could generate more suitable micro-environmental conditions for the establishment of other plants like Colobanthus quitensis (Cq). Although positive plant–plant interactions have been shown to contribute to plant performance and establishment, little is known about how microorganisms might modulate those interactions, particularly in stressful environmental conditions. Several reports have focused on the possible ecological roles of microorganisms on vascular plants, but if rhizospheric microorganisms can impact positive interactions among Antarctic plants has been seldom studied. Here, we assessed the physical–chemical characteristics of rhizospheric soils from Cq growing alone or associated with Da (Cq + Da). In addition, we compared the rhizosphere microbiomes associated with Cq, either growing alone or associated with Da (Cq + Da), using a shotgun metagenomic DNA sequencing approach and using eggNOG for comparative and functional metagenomics. Overall, there were no differences among rhizospheric soils in terms of physical–chemical characteristics. On the other hand, our results show significant differences in terms of taxonomic diversity between rhizospheric soils. Functional annotation and pathway analysis showed that microorganisms from rhizospheric soil samples also have significant differences in gene abundance associated with several functional categories related to environmental tolerance and in metabolic pathways linked to osmotic stress, among others. Overall, this study provides foundational information which will allow to explore the biological impact of the rhizobiome and its functional mechanisms and molecular pathways on plant performance and help explain the concerted strategy deployed by Cq to inhabit and cope with the harsh conditions prevailing in Antarctica.",
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A first insight into the structure and function of rhizosphere microbiota in Antarctic plants using shotgun metagenomic. / Molina-Montenegro, Marco A.; Ballesteros, Gabriel I.; Castro-Nallar, Eduardo; Meneses, Claudio; Gallardo-Cerda, Jorge; Torres-Díaz, Cristian.

En: Polar Biology, 01.01.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - A first insight into the structure and function of rhizosphere microbiota in Antarctic plants using shotgun metagenomic

AU - Molina-Montenegro, Marco A.

AU - Ballesteros, Gabriel I.

AU - Castro-Nallar, Eduardo

AU - Meneses, Claudio

AU - Gallardo-Cerda, Jorge

AU - Torres-Díaz, Cristian

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Antarctic vascular plants such as Deschampsia antarctica (Da) could generate more suitable micro-environmental conditions for the establishment of other plants like Colobanthus quitensis (Cq). Although positive plant–plant interactions have been shown to contribute to plant performance and establishment, little is known about how microorganisms might modulate those interactions, particularly in stressful environmental conditions. Several reports have focused on the possible ecological roles of microorganisms on vascular plants, but if rhizospheric microorganisms can impact positive interactions among Antarctic plants has been seldom studied. Here, we assessed the physical–chemical characteristics of rhizospheric soils from Cq growing alone or associated with Da (Cq + Da). In addition, we compared the rhizosphere microbiomes associated with Cq, either growing alone or associated with Da (Cq + Da), using a shotgun metagenomic DNA sequencing approach and using eggNOG for comparative and functional metagenomics. Overall, there were no differences among rhizospheric soils in terms of physical–chemical characteristics. On the other hand, our results show significant differences in terms of taxonomic diversity between rhizospheric soils. Functional annotation and pathway analysis showed that microorganisms from rhizospheric soil samples also have significant differences in gene abundance associated with several functional categories related to environmental tolerance and in metabolic pathways linked to osmotic stress, among others. Overall, this study provides foundational information which will allow to explore the biological impact of the rhizobiome and its functional mechanisms and molecular pathways on plant performance and help explain the concerted strategy deployed by Cq to inhabit and cope with the harsh conditions prevailing in Antarctica.

AB - Antarctic vascular plants such as Deschampsia antarctica (Da) could generate more suitable micro-environmental conditions for the establishment of other plants like Colobanthus quitensis (Cq). Although positive plant–plant interactions have been shown to contribute to plant performance and establishment, little is known about how microorganisms might modulate those interactions, particularly in stressful environmental conditions. Several reports have focused on the possible ecological roles of microorganisms on vascular plants, but if rhizospheric microorganisms can impact positive interactions among Antarctic plants has been seldom studied. Here, we assessed the physical–chemical characteristics of rhizospheric soils from Cq growing alone or associated with Da (Cq + Da). In addition, we compared the rhizosphere microbiomes associated with Cq, either growing alone or associated with Da (Cq + Da), using a shotgun metagenomic DNA sequencing approach and using eggNOG for comparative and functional metagenomics. Overall, there were no differences among rhizospheric soils in terms of physical–chemical characteristics. On the other hand, our results show significant differences in terms of taxonomic diversity between rhizospheric soils. Functional annotation and pathway analysis showed that microorganisms from rhizospheric soil samples also have significant differences in gene abundance associated with several functional categories related to environmental tolerance and in metabolic pathways linked to osmotic stress, among others. Overall, this study provides foundational information which will allow to explore the biological impact of the rhizobiome and its functional mechanisms and molecular pathways on plant performance and help explain the concerted strategy deployed by Cq to inhabit and cope with the harsh conditions prevailing in Antarctica.

KW - Functional symbiosis

KW - Gene ontology

KW - Rhizobiome

KW - Vascular antarctic plants

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U2 - 10.1007/s00300-019-02556-7

DO - 10.1007/s00300-019-02556-7

M3 - Article

JO - Polar Biology

JF - Polar Biology

SN - 0722-4060

ER -