A comparative study of structure and function of the longitudinal muscle of the anal canal and the internal anal sphincter in pigs

Alvaro Opazo, Begoña Lecea, Carme Admella, Maria José Fantova, Marcel Jiménez, Joan Martí-Ragué, Pere Clavé

Resultado de la investigación: Article

6 Citas (Scopus)

Resumen

PURPOSE: This study aims to compare the physiology of the longitudinal muscle of the anal canal with the internal anal sphincter in pigs. METHODS: Histology and in vitro studies were performed to compare the effect of neural responses induced by electric stimulation and through nicotinic, purinergic, and serotoninergic receptors. RESULTS: The longitudinal muscle and the internal anal sphincter are constituted exclusively by smooth muscle. (Strips from the internal anal sphincter a) (developed myogenic tone; b) (responded to electric stimulation with an "on" relaxation antagonized by nitric oxide synthase inhibitors and purinergic P2Y1 antagonists, and with an "off contraction antagonized by atropine and phentolamine; and c) (responded to stimulation of nicotinic receptors with a relaxation antagonized by nitrergic and purinergic P2Y1 antagonists, responded to stimulation of serotoninergic 5-hydroxytryptamine 3 receptors with a contraction, and relaxed to carbachol and purinergic P2X agonists. Strips from the longitudinal muscle a) (did not develop tone, b) (responded to electric stimulation with an "on" contraction antagonized by atropine, and c) did not respond to stimulation of nicotinic or serotoninergic 5-hydroxytryptamine 3 receptors, and carbachol and purinergic P2X agonists induced a contraction. CONCLUSIONS: The motility of the internal anal sphincter includes myogenic tone, relaxation mediated by nitric oxide and purinergic P2Y1 receptors, and contraction mediated by cholinergic motor neurons and sympathetic fibers. The motility of the longitudinal muscle is limited to a contraction mediated by cholinergic neurons, suggesting that longitudinal muscle contracts during relaxation of the internal sphincter, shortening the anal canal. Nicotinic, muscarinic, and serotoninergic receptors might be therapeutic targets for anal motor disorders.

Idioma originalEnglish
Páginas (desde-hasta)1902-1911
Número de páginas10
PublicaciónDiseases of the Colon and Rectum
Volumen52
N.º11
DOI
EstadoPublished - 1 nov 2009

Huella dactilar

Anal Canal
Swine
Muscles
Purinergic Antagonists
Purinergic Agonists
Electric Stimulation
Receptors, Serotonin, 5-HT3
Cholinergic Neurons
Carbachol
Atropine
Purinergic P2Y1 Receptors
Adrenergic Fibers
Purinergic Receptors
Phentolamine
Nicotinic Receptors
Motor Neurons
Muscarinic Receptors
Nitric Oxide Synthase
Smooth Muscle
Histology

ASJC Scopus subject areas

  • Gastroenterology

Citar esto

Opazo, Alvaro ; Lecea, Begoña ; Admella, Carme ; Fantova, Maria José ; Jiménez, Marcel ; Martí-Ragué, Joan ; Clavé, Pere. / A comparative study of structure and function of the longitudinal muscle of the anal canal and the internal anal sphincter in pigs. En: Diseases of the Colon and Rectum. 2009 ; Vol. 52, N.º 11. pp. 1902-1911.
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title = "A comparative study of structure and function of the longitudinal muscle of the anal canal and the internal anal sphincter in pigs",
abstract = "PURPOSE: This study aims to compare the physiology of the longitudinal muscle of the anal canal with the internal anal sphincter in pigs. METHODS: Histology and in vitro studies were performed to compare the effect of neural responses induced by electric stimulation and through nicotinic, purinergic, and serotoninergic receptors. RESULTS: The longitudinal muscle and the internal anal sphincter are constituted exclusively by smooth muscle. (Strips from the internal anal sphincter a) (developed myogenic tone; b) (responded to electric stimulation with an {"}on{"} relaxation antagonized by nitric oxide synthase inhibitors and purinergic P2Y1 antagonists, and with an {"}off contraction antagonized by atropine and phentolamine; and c) (responded to stimulation of nicotinic receptors with a relaxation antagonized by nitrergic and purinergic P2Y1 antagonists, responded to stimulation of serotoninergic 5-hydroxytryptamine 3 receptors with a contraction, and relaxed to carbachol and purinergic P2X agonists. Strips from the longitudinal muscle a) (did not develop tone, b) (responded to electric stimulation with an {"}on{"} contraction antagonized by atropine, and c) did not respond to stimulation of nicotinic or serotoninergic 5-hydroxytryptamine 3 receptors, and carbachol and purinergic P2X agonists induced a contraction. CONCLUSIONS: The motility of the internal anal sphincter includes myogenic tone, relaxation mediated by nitric oxide and purinergic P2Y1 receptors, and contraction mediated by cholinergic motor neurons and sympathetic fibers. The motility of the longitudinal muscle is limited to a contraction mediated by cholinergic neurons, suggesting that longitudinal muscle contracts during relaxation of the internal sphincter, shortening the anal canal. Nicotinic, muscarinic, and serotoninergic receptors might be therapeutic targets for anal motor disorders.",
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author = "Alvaro Opazo and Bego{\~n}a Lecea and Carme Admella and Fantova, {Maria Jos{\'e}} and Marcel Jim{\'e}nez and Joan Mart{\'i}-Ragu{\'e} and Pere Clav{\'e}",
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A comparative study of structure and function of the longitudinal muscle of the anal canal and the internal anal sphincter in pigs. / Opazo, Alvaro; Lecea, Begoña; Admella, Carme; Fantova, Maria José; Jiménez, Marcel; Martí-Ragué, Joan; Clavé, Pere.

En: Diseases of the Colon and Rectum, Vol. 52, N.º 11, 01.11.2009, p. 1902-1911.

Resultado de la investigación: Article

TY - JOUR

T1 - A comparative study of structure and function of the longitudinal muscle of the anal canal and the internal anal sphincter in pigs

AU - Opazo, Alvaro

AU - Lecea, Begoña

AU - Admella, Carme

AU - Fantova, Maria José

AU - Jiménez, Marcel

AU - Martí-Ragué, Joan

AU - Clavé, Pere

PY - 2009/11/1

Y1 - 2009/11/1

N2 - PURPOSE: This study aims to compare the physiology of the longitudinal muscle of the anal canal with the internal anal sphincter in pigs. METHODS: Histology and in vitro studies were performed to compare the effect of neural responses induced by electric stimulation and through nicotinic, purinergic, and serotoninergic receptors. RESULTS: The longitudinal muscle and the internal anal sphincter are constituted exclusively by smooth muscle. (Strips from the internal anal sphincter a) (developed myogenic tone; b) (responded to electric stimulation with an "on" relaxation antagonized by nitric oxide synthase inhibitors and purinergic P2Y1 antagonists, and with an "off contraction antagonized by atropine and phentolamine; and c) (responded to stimulation of nicotinic receptors with a relaxation antagonized by nitrergic and purinergic P2Y1 antagonists, responded to stimulation of serotoninergic 5-hydroxytryptamine 3 receptors with a contraction, and relaxed to carbachol and purinergic P2X agonists. Strips from the longitudinal muscle a) (did not develop tone, b) (responded to electric stimulation with an "on" contraction antagonized by atropine, and c) did not respond to stimulation of nicotinic or serotoninergic 5-hydroxytryptamine 3 receptors, and carbachol and purinergic P2X agonists induced a contraction. CONCLUSIONS: The motility of the internal anal sphincter includes myogenic tone, relaxation mediated by nitric oxide and purinergic P2Y1 receptors, and contraction mediated by cholinergic motor neurons and sympathetic fibers. The motility of the longitudinal muscle is limited to a contraction mediated by cholinergic neurons, suggesting that longitudinal muscle contracts during relaxation of the internal sphincter, shortening the anal canal. Nicotinic, muscarinic, and serotoninergic receptors might be therapeutic targets for anal motor disorders.

AB - PURPOSE: This study aims to compare the physiology of the longitudinal muscle of the anal canal with the internal anal sphincter in pigs. METHODS: Histology and in vitro studies were performed to compare the effect of neural responses induced by electric stimulation and through nicotinic, purinergic, and serotoninergic receptors. RESULTS: The longitudinal muscle and the internal anal sphincter are constituted exclusively by smooth muscle. (Strips from the internal anal sphincter a) (developed myogenic tone; b) (responded to electric stimulation with an "on" relaxation antagonized by nitric oxide synthase inhibitors and purinergic P2Y1 antagonists, and with an "off contraction antagonized by atropine and phentolamine; and c) (responded to stimulation of nicotinic receptors with a relaxation antagonized by nitrergic and purinergic P2Y1 antagonists, responded to stimulation of serotoninergic 5-hydroxytryptamine 3 receptors with a contraction, and relaxed to carbachol and purinergic P2X agonists. Strips from the longitudinal muscle a) (did not develop tone, b) (responded to electric stimulation with an "on" contraction antagonized by atropine, and c) did not respond to stimulation of nicotinic or serotoninergic 5-hydroxytryptamine 3 receptors, and carbachol and purinergic P2X agonists induced a contraction. CONCLUSIONS: The motility of the internal anal sphincter includes myogenic tone, relaxation mediated by nitric oxide and purinergic P2Y1 receptors, and contraction mediated by cholinergic motor neurons and sympathetic fibers. The motility of the longitudinal muscle is limited to a contraction mediated by cholinergic neurons, suggesting that longitudinal muscle contracts during relaxation of the internal sphincter, shortening the anal canal. Nicotinic, muscarinic, and serotoninergic receptors might be therapeutic targets for anal motor disorders.

KW - Anal longitudinal muscle

KW - Enteric motor neurons

KW - Inhibitory neurotransmission

KW - Internal anal sphincter

KW - Nicotinic receptors

KW - Purinergic receptors

KW - Serotoninergic receptors

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U2 - 10.1007/DCR.0b013e3181b160be

DO - 10.1007/DCR.0b013e3181b160be

M3 - Article

C2 - 19966640

AN - SCOPUS:75349099906

VL - 52

SP - 1902

EP - 1911

JO - Diseases of the Colon and Rectum

JF - Diseases of the Colon and Rectum

SN - 0012-3706

IS - 11

ER -