A codominant diagnostic marker for the slow ripening trait in peach

Claudio Meneses, Lissette Ulloa-Zepeda, Alejandra Cifuentes-Esquivel, Rodrigo Infante, Celia M. Cantin, Ignasi Batlle, Pere Arús, Iban Eduardo

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

The peach [Prunus persica L. (Batsch)] slow ripening (SR) trait is a mutation preventing the normal ripening process. Individuals with this phenotype are discarded in peach breeding programs. This trait is determined by a single gene (Sr/sr), where the recessive homozygote (sr/sr) confers the SR phenotype, and has been mapped to linkage group 4 of the peach genome. A large deletion of 26.6 kb containing the sequence of a NAC transcription factor has been proposed as the causal mutation. Two dominant markers based on the sequence of this region have been assayed previously and found to be diagnostic (genotypes always predicted the phenotypes). However, their dominant nature—a null allele for the marker was associated with the sr allele—made it impossible to predict the individuals that carried the SR trait. Here we used resequencing information to develop a codominant molecular marker for the SR trait in peach. The marker was validated in the ‘Belbinette’ × ‘Nectalady’ F1 and the ‘Venus’ F2 populations, and in 27 lines, 18 of which are known to carry the sr allele. The marker cosegregated with the SR phenotype in all cases, allowing the discrimination of two DNA fragments of different size associated with normal-ripening alleles, in addition to a third fragment associated with the sr allele. The utility of this marker in peach breeding programs is discussed.

Idioma originalEnglish
Número de artículo77
PublicaciónMolecular Breeding
Volumen36
N.º6
DOI
EstadoPublished - 1 jun 2016

Huella dactilar

peaches
ripening
Alleles
Phenotype
phenotype
Breeding
alleles
Venus
Mutation
mutation
Homozygote
null alleles
Prunus persica
breeding
homozygosity
linkage groups
Transcription Factors
Genotype
Genome
transcription factors

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Citar esto

Meneses, C., Ulloa-Zepeda, L., Cifuentes-Esquivel, A., Infante, R., Cantin, C. M., Batlle, I., ... Eduardo, I. (2016). A codominant diagnostic marker for the slow ripening trait in peach. Molecular Breeding, 36(6), [77]. https://doi.org/10.1007/s11032-016-0506-7
Meneses, Claudio ; Ulloa-Zepeda, Lissette ; Cifuentes-Esquivel, Alejandra ; Infante, Rodrigo ; Cantin, Celia M. ; Batlle, Ignasi ; Arús, Pere ; Eduardo, Iban. / A codominant diagnostic marker for the slow ripening trait in peach. En: Molecular Breeding. 2016 ; Vol. 36, N.º 6.
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abstract = "The peach [Prunus persica L. (Batsch)] slow ripening (SR) trait is a mutation preventing the normal ripening process. Individuals with this phenotype are discarded in peach breeding programs. This trait is determined by a single gene (Sr/sr), where the recessive homozygote (sr/sr) confers the SR phenotype, and has been mapped to linkage group 4 of the peach genome. A large deletion of 26.6 kb containing the sequence of a NAC transcription factor has been proposed as the causal mutation. Two dominant markers based on the sequence of this region have been assayed previously and found to be diagnostic (genotypes always predicted the phenotypes). However, their dominant nature—a null allele for the marker was associated with the sr allele—made it impossible to predict the individuals that carried the SR trait. Here we used resequencing information to develop a codominant molecular marker for the SR trait in peach. The marker was validated in the ‘Belbinette’ × ‘Nectalady’ F1 and the ‘Venus’ F2 populations, and in 27 lines, 18 of which are known to carry the sr allele. The marker cosegregated with the SR phenotype in all cases, allowing the discrimination of two DNA fragments of different size associated with normal-ripening alleles, in addition to a third fragment associated with the sr allele. The utility of this marker in peach breeding programs is discussed.",
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Meneses, C, Ulloa-Zepeda, L, Cifuentes-Esquivel, A, Infante, R, Cantin, CM, Batlle, I, Arús, P & Eduardo, I 2016, 'A codominant diagnostic marker for the slow ripening trait in peach', Molecular Breeding, vol. 36, n.º 6, 77. https://doi.org/10.1007/s11032-016-0506-7

A codominant diagnostic marker for the slow ripening trait in peach. / Meneses, Claudio; Ulloa-Zepeda, Lissette; Cifuentes-Esquivel, Alejandra; Infante, Rodrigo; Cantin, Celia M.; Batlle, Ignasi; Arús, Pere; Eduardo, Iban.

En: Molecular Breeding, Vol. 36, N.º 6, 77, 01.06.2016.

Resultado de la investigación: Article

TY - JOUR

T1 - A codominant diagnostic marker for the slow ripening trait in peach

AU - Meneses, Claudio

AU - Ulloa-Zepeda, Lissette

AU - Cifuentes-Esquivel, Alejandra

AU - Infante, Rodrigo

AU - Cantin, Celia M.

AU - Batlle, Ignasi

AU - Arús, Pere

AU - Eduardo, Iban

PY - 2016/6/1

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N2 - The peach [Prunus persica L. (Batsch)] slow ripening (SR) trait is a mutation preventing the normal ripening process. Individuals with this phenotype are discarded in peach breeding programs. This trait is determined by a single gene (Sr/sr), where the recessive homozygote (sr/sr) confers the SR phenotype, and has been mapped to linkage group 4 of the peach genome. A large deletion of 26.6 kb containing the sequence of a NAC transcription factor has been proposed as the causal mutation. Two dominant markers based on the sequence of this region have been assayed previously and found to be diagnostic (genotypes always predicted the phenotypes). However, their dominant nature—a null allele for the marker was associated with the sr allele—made it impossible to predict the individuals that carried the SR trait. Here we used resequencing information to develop a codominant molecular marker for the SR trait in peach. The marker was validated in the ‘Belbinette’ × ‘Nectalady’ F1 and the ‘Venus’ F2 populations, and in 27 lines, 18 of which are known to carry the sr allele. The marker cosegregated with the SR phenotype in all cases, allowing the discrimination of two DNA fragments of different size associated with normal-ripening alleles, in addition to a third fragment associated with the sr allele. The utility of this marker in peach breeding programs is discussed.

AB - The peach [Prunus persica L. (Batsch)] slow ripening (SR) trait is a mutation preventing the normal ripening process. Individuals with this phenotype are discarded in peach breeding programs. This trait is determined by a single gene (Sr/sr), where the recessive homozygote (sr/sr) confers the SR phenotype, and has been mapped to linkage group 4 of the peach genome. A large deletion of 26.6 kb containing the sequence of a NAC transcription factor has been proposed as the causal mutation. Two dominant markers based on the sequence of this region have been assayed previously and found to be diagnostic (genotypes always predicted the phenotypes). However, their dominant nature—a null allele for the marker was associated with the sr allele—made it impossible to predict the individuals that carried the SR trait. Here we used resequencing information to develop a codominant molecular marker for the SR trait in peach. The marker was validated in the ‘Belbinette’ × ‘Nectalady’ F1 and the ‘Venus’ F2 populations, and in 27 lines, 18 of which are known to carry the sr allele. The marker cosegregated with the SR phenotype in all cases, allowing the discrimination of two DNA fragments of different size associated with normal-ripening alleles, in addition to a third fragment associated with the sr allele. The utility of this marker in peach breeding programs is discussed.

KW - Fruit quality

KW - Marker-assisted selection

KW - Molecular marker

KW - Prunus persica

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VL - 36

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

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Meneses C, Ulloa-Zepeda L, Cifuentes-Esquivel A, Infante R, Cantin CM, Batlle I y otros. A codominant diagnostic marker for the slow ripening trait in peach. Molecular Breeding. 2016 jun 1;36(6). 77. https://doi.org/10.1007/s11032-016-0506-7