α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

A. J. Brake; J. P. Merryweather; D. G. Coit; U. A. Heberlein; F. R. Masiarz; G. T. Mullenbach; M. S. Urdea; P. Valenzuela; P. J. Barr

doi:10.1073/pnas.81.15.4642

α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

A. J. Brake, J. P. Merryweather, D. G. Coit, U. A. Heberlein, F. R. Masiarz, G. T. Mullenbach, M. S. Urdea, P. Valenzuela, P. J. Barr

Facultad de Ciencias de la Vida

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

400 Citas (Scopus)

Resumen

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

Idioma original	Inglés
Páginas (desde-hasta)	4642-4646
Número de páginas	5
Publicación	Proceedings of the National Academy of Sciences of the United States of America
Volumen	81
N.º	15 I
DOI	https://doi.org/10.1073/pnas.81.15.4642
Estado	Publicada - 1984

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Brake, A. J., Merryweather, J. P., Coit, D. G., Heberlein, U. A., Masiarz, F. R., Mullenbach, G. T., Urdea, M. S., Valenzuela, P., & Barr, P. J. (1984). α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America, 81(15 I), 4642-4646. https://doi.org/10.1073/pnas.81.15.4642

Brake, A. J. ; Merryweather, J. P. ; Coit, D. G. ; Heberlein, U. A. ; Masiarz, F. R. ; Mullenbach, G. T. ; Urdea, M. S. ; Valenzuela, P. ; Barr, P. J. / α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. En: Proceedings of the National Academy of Sciences of the United States of America. 1984 ; Vol. 81, N.º 15 I. pp. 4642-4646.

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title = "α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae",

abstract = "Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.",

author = "Brake, {A. J.} and Merryweather, {J. P.} and Coit, {D. G.} and Heberlein, {U. A.} and Masiarz, {F. R.} and Mullenbach, {G. T.} and Urdea, {M. S.} and P. Valenzuela and Barr, {P. J.}",

year = "1984",

doi = "10.1073/pnas.81.15.4642",

language = "English",

volume = "81",

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journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Brake, AJ, Merryweather, JP, Coit, DG, Heberlein, UA, Masiarz, FR, Mullenbach, GT, Urdea, MS, Valenzuela, P & Barr, PJ 1984, 'α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae', Proceedings of the National Academy of Sciences of the United States of America, vol. 81, n.º 15 I, pp. 4642-4646. https://doi.org/10.1073/pnas.81.15.4642

α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. / Brake, A. J.; Merryweather, J. P.; Coit, D. G.; Heberlein, U. A.; Masiarz, F. R.; Mullenbach, G. T.; Urdea, M. S.; Valenzuela, P.; Barr, P. J.

En: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, N.º 15 I, 1984, p. 4642-4646.

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

AU - Brake, A. J.

AU - Merryweather, J. P.

AU - Coit, D. G.

AU - Heberlein, U. A.

AU - Masiarz, F. R.

AU - Mullenbach, G. T.

AU - Urdea, M. S.

AU - Valenzuela, P.

AU - Barr, P. J.

PY - 1984

Y1 - 1984

N2 - Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

AB - Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

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DO - 10.1073/pnas.81.15.4642

M3 - Article

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AN - SCOPUS:0000372323

VL - 81

SP - 4642

EP - 4646

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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IS - 15 I

ER -

α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

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