α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

A. J. Brake, J. P. Merryweather, D. G. Coit, U. A. Heberlein, F. R. Masiarz, G. T. Mullenbach, M. S. Urdea, P. Valenzuela, P. J. Barr

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

390 Citas (Scopus)

Resumen

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

Idioma originalInglés
Páginas (desde-hasta)4642-4646
Número de páginas5
PublicaciónProceedings of the National Academy of Sciences of the United States of America
Volumen81
N.º15 I
DOI
EstadoPublicada - 1984

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