TY - JOUR
T1 - Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis
AU - Perrin, Robyn M.
AU - DeRocher, Amy E.
AU - Bar-Peled, Maor
AU - Zeng, Weiqing
AU - Norambuena, Lorena
AU - Orellana, Ariel
AU - Raikhel, Natasha V.
AU - Keegstra, Kenneth
PY - 1999/6/18
Y1 - 1999/6/18
N2 - Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, AtFT1, from Arabidopsis. Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized Arabidopsis membrane proteins, and AtFT1 expressed in mammalian COS cells results in the presence of XG FTase activity in these cells.
AB - Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, AtFT1, from Arabidopsis. Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized Arabidopsis membrane proteins, and AtFT1 expressed in mammalian COS cells results in the presence of XG FTase activity in these cells.
UR - http://www.scopus.com/inward/record.url?scp=0033580981&partnerID=8YFLogxK
U2 - 10.1126/science.284.5422.1976
DO - 10.1126/science.284.5422.1976
M3 - Article
C2 - 10373113
AN - SCOPUS:0033580981
SN - 0036-8075
VL - 284
SP - 1976
EP - 1979
JO - Science
JF - Science
IS - 5422
ER -