Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection

Jimena Gatica, Paula I. Rodas, Alejandro Escobar

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Macrophages are critical cells in the innate immune response to microorganisms sensed in the tissues. During infections, the interaction between pathogens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, Neisseria gonorrhoeae can suppress the protective immune response at this level, avoiding its detection and elimination. Studies addressed to develop the interactions between macrophages and Neisseria gonorrhoeae allow us to find potential targets to be exploited with vaccines and therapeutic drugs. In this chapter, we describe protocols to generate human monocyte-derived macrophages and assess their response to infection with Neisseria gonorrhoeae.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages301-318
Number of pages18
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1997
ISSN (Print)1064-3745

Keywords

  • Cytokines
  • Monocyte-derived macrophages
  • Neisseria gonorrhoeae
  • Phagocytosis
  • T cell proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Gatica, J., Rodas, P. I., & Escobar, A. (2019). Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection. In Methods in Molecular Biology (pp. 301-318). (Methods in Molecular Biology; Vol. 1997). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9496-0_18