Two families of repeated DNA sequences in Thiobacillus ferrooxidans

J. R. Yates, D. S. Holmes

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

The genome of Thiobacillus ferrooxidans ATCC 19859 is about 2.8 x 106 base pairs as determined by analysis of reassociation kinetics of sheared DNA. This is 70% of the size of the genome of Escherichia coli. About 6% of the genome of T. ferrooxidans consists of moderately repetitive DNA sequences that are repeated an average of 20 times per genome. Two distinct repeated sequences, designated family 1 and family 2, have been analyzed in more detail. Both families are approximately 1 kilobase in length and are repeated 20 to 30 times per genome. Preliminary evidence from restriction enzyme analysis, Southern blotting experiments, and thermal melting analysis indicates that members of both families are conserved and are interspersed with single-copy DNA. Six copies of one family are present on the 45-kilobase-pair plasmid of strain ATCC 19859.

Original languageEnglish
Pages (from-to)1861-1870
Number of pages10
JournalJournal of Bacteriology
Volume169
Issue number5
DOIs
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Two families of repeated DNA sequences in Thiobacillus ferrooxidans'. Together they form a unique fingerprint.

Cite this