Transcriptomics using next generation sequencing technologies

Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faunes, Francisco Melo, Juan Larrain

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Citations (Scopus)

Abstract

Next generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.

Original languageEnglish
Title of host publicationXenopus Protocols
Pages293-317
Number of pages25
Volume917
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume917
ISSN (Print)10643745

Keywords

  • High-throughput sequencing
  • Illumina
  • Massively -parallel sequencing
  • Next generation sequencing
  • RNA-Seq
  • Small RNA-Seq
  • Transcriptional profiling
  • Transcriptomics
  • Xenopus laevis
  • Xenopus tropicalis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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