TY - JOUR
T1 - Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco
AU - Daurelio, Lucas Damián
AU - Petrocelli, Silvana
AU - Blanco, Francisca
AU - Holuigue, Loreto
AU - Ottado, Jorgelina
AU - Orellano, Elena Graciela
N1 - Funding Information:
This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica ( ANPCyT PICT 01-12783 to EGO) and from CONICYT, Chile ( FONDECYT 1060494 to L.H.). EGO and JO are staff members and LDD and SP are Fellows of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina).
Funding Information:
The authors particularly thank the AMSUD Pasteur program for funding LDD's stay at the Pontificia Universidad Católica de Chile. We are also grateful to Dr. Estela M. Valle and Dr. Daniela V. Rial for providing corrections and making very important contributions to this manuscript.
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the nonhost response, which is usually accompanied by transcriptional changes. In order to identify genes involved in nonhost resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a nonhost phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24. h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20% of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with nonhost resistance in plant-pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for nonhost disease resistance.
AB - Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the nonhost response, which is usually accompanied by transcriptional changes. In order to identify genes involved in nonhost resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a nonhost phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24. h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20% of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with nonhost resistance in plant-pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for nonhost disease resistance.
KW - CDNA-AFLP transcriptome analysis
KW - Hypersensitive response
KW - Nicotiana tabacum
KW - Nonhost response
KW - Xanthomonas axonopodis pv. citri
UR - http://www.scopus.com/inward/record.url?scp=78651426450&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2010.07.014
DO - 10.1016/j.jplph.2010.07.014
M3 - Article
C2 - 20828873
AN - SCOPUS:78651426450
SN - 0176-1617
VL - 168
SP - 382
EP - 391
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 4
ER -