Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp

Gudrun Kausel, María Inés Vera, Rody San Martin, Jaime Figueroa, Alfredo Molina, Marc Muller, Joseph Martial, Manuel Krauskopf

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.

Original languageEnglish
Pages (from-to)598-609
Number of pages12
JournalJournal of Cellular Biochemistry
Volume75
Issue number4
DOIs
Publication statusPublished - 15 Dec 1999

Keywords

  • Acclimatization
  • Carp
  • Fish
  • Genomic organization
  • Pit-1
  • Pit-1 gene expression
  • Seasonal control

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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