The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells

Roberto Paredes; Gloria Arriagada; Fernando Cruzat; Juan Olate; Andre Van Wijnen; Jane Lian; Gary Stein; Janet Stein; Martin Montecino

doi:10.1016/j.jsbmb.2004.03.076

The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells

Roberto Paredes, Gloria Arriagada, Fernando Cruzat, Juan Olate, Andre Van Wijnen, Jane Lian, Gary Stein, Janet Stein, Martin Montecino

Life Sciences School

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Bone-specific transcription of the osteocalcin (OC) gene is principally regulated by the Runx2 transcription factor and further stimulated in response to 1α,25-dihydroxy Vitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A-C). Mutation of sites A and B, which flank the 1α,25-dihydroxy Vitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxy Vitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between VDR and Runx2 factors. Additionally, the transcriptional co-activator p300 is recruited to the OC promoter by Runx2 where it up-regulates both basal and 1α,25-dihydroxy Vitamin D3-enhanced OC expression. Here, we present an overview of how in osteoblastic cells expressing OC, Runx2 modulates the 1α,25-dihydroxy Vitamin D3-dependent stimulation of the OC promoter by first recruiting transcriptional co-activators and then by further stabilizing the interaction of the VDR with the VDRE.

Original language	English
Pages (from-to)	269-271
Number of pages	3
Journal	Journal of Steroid Biochemistry and Molecular Biology
Volume	89-90
DOIs	https://doi.org/10.1016/j.jsbmb.2004.03.076
Publication status	Published - May 2004

Keywords

Osteocalcin
Runx2
Transcription
Vitamin D

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Medicine
Molecular Biology
Endocrinology
Clinical Biochemistry
Cell Biology

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10.1016/j.jsbmb.2004.03.076

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Paredes, R., Arriagada, G., Cruzat, F., Olate, J., Van Wijnen, A., Lian, J., Stein, G., Stein, J., & Montecino, M. (2004). The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells. Journal of Steroid Biochemistry and Molecular Biology, 89-90, 269-271. https://doi.org/10.1016/j.jsbmb.2004.03.076

@article{3988beef508e4d4da998a15e8b1eab8a,

title = "The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells",

abstract = "Bone-specific transcription of the osteocalcin (OC) gene is principally regulated by the Runx2 transcription factor and further stimulated in response to 1α,25-dihydroxy Vitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A-C). Mutation of sites A and B, which flank the 1α,25-dihydroxy Vitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxy Vitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between VDR and Runx2 factors. Additionally, the transcriptional co-activator p300 is recruited to the OC promoter by Runx2 where it up-regulates both basal and 1α,25-dihydroxy Vitamin D3-enhanced OC expression. Here, we present an overview of how in osteoblastic cells expressing OC, Runx2 modulates the 1α,25-dihydroxy Vitamin D3-dependent stimulation of the OC promoter by first recruiting transcriptional co-activators and then by further stabilizing the interaction of the VDR with the VDRE.",

keywords = "Osteocalcin, Runx2, Transcription, Vitamin D",

author = "Roberto Paredes and Gloria Arriagada and Fernando Cruzat and Juan Olate and {Van Wijnen}, Andre and Jane Lian and Gary Stein and Janet Stein and Martin Montecino",

note = "Funding Information: We thank Drs. Jose Gutierrez and Soraya Gutierrez for helpful discussions and critical reading of this manuscript. This work was supported by grants from FONDECYT 1030749 (to M.M.) and 2010103 (to R.P.), NIH-FIRCA TW00990 (to G.S. and M.M.), DIUC 201.031.090-1.4 (to M.M.), and NIH AR48818 (to G.S.). F.C. has been supported by a fellowship from MECESUP UCH9903 and R.P. by a scholarship from Fundacion Andes. The contents are solely responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health.",

year = "2004",

month = may,

doi = "10.1016/j.jsbmb.2004.03.076",

language = "English",

volume = "89-90",

pages = "269--271",

journal = "Journal of Steroid Biochemistry and Molecular Biology",

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Paredes, R, Arriagada, G, Cruzat, F, Olate, J, Van Wijnen, A, Lian, J, Stein, G, Stein, J & Montecino, M 2004, 'The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells', Journal of Steroid Biochemistry and Molecular Biology, vol. 89-90, pp. 269-271. https://doi.org/10.1016/j.jsbmb.2004.03.076

The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells. / Paredes, Roberto; Arriagada, Gloria; Cruzat, Fernando et al.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 89-90, 05.2004, p. 269-271.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells

AU - Paredes, Roberto

AU - Arriagada, Gloria

AU - Cruzat, Fernando

AU - Olate, Juan

AU - Van Wijnen, Andre

AU - Lian, Jane

AU - Stein, Gary

AU - Stein, Janet

AU - Montecino, Martin

N1 - Funding Information: We thank Drs. Jose Gutierrez and Soraya Gutierrez for helpful discussions and critical reading of this manuscript. This work was supported by grants from FONDECYT 1030749 (to M.M.) and 2010103 (to R.P.), NIH-FIRCA TW00990 (to G.S. and M.M.), DIUC 201.031.090-1.4 (to M.M.), and NIH AR48818 (to G.S.). F.C. has been supported by a fellowship from MECESUP UCH9903 and R.P. by a scholarship from Fundacion Andes. The contents are solely responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health.

PY - 2004/5

Y1 - 2004/5

N2 - Bone-specific transcription of the osteocalcin (OC) gene is principally regulated by the Runx2 transcription factor and further stimulated in response to 1α,25-dihydroxy Vitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A-C). Mutation of sites A and B, which flank the 1α,25-dihydroxy Vitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxy Vitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between VDR and Runx2 factors. Additionally, the transcriptional co-activator p300 is recruited to the OC promoter by Runx2 where it up-regulates both basal and 1α,25-dihydroxy Vitamin D3-enhanced OC expression. Here, we present an overview of how in osteoblastic cells expressing OC, Runx2 modulates the 1α,25-dihydroxy Vitamin D3-dependent stimulation of the OC promoter by first recruiting transcriptional co-activators and then by further stabilizing the interaction of the VDR with the VDRE.

AB - Bone-specific transcription of the osteocalcin (OC) gene is principally regulated by the Runx2 transcription factor and further stimulated in response to 1α,25-dihydroxy Vitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A-C). Mutation of sites A and B, which flank the 1α,25-dihydroxy Vitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxy Vitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between VDR and Runx2 factors. Additionally, the transcriptional co-activator p300 is recruited to the OC promoter by Runx2 where it up-regulates both basal and 1α,25-dihydroxy Vitamin D3-enhanced OC expression. Here, we present an overview of how in osteoblastic cells expressing OC, Runx2 modulates the 1α,25-dihydroxy Vitamin D3-dependent stimulation of the OC promoter by first recruiting transcriptional co-activators and then by further stabilizing the interaction of the VDR with the VDRE.

KW - Osteocalcin

KW - Runx2

KW - Transcription

KW - Vitamin D

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DO - 10.1016/j.jsbmb.2004.03.076

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SN - 0960-0760

VL - 89-90

SP - 269

EP - 271

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

ER -

The Runx2 transcription factor plays a key role in the 1α,25- dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells

Abstract

Keywords

ASJC Scopus subject areas

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