The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance

Cecilia Rodriguez-Furlán, Hernán Salinas-Grenet, Omar Sandoval, Camilo Recabarren, Paulina Arraño-Salinas, Sylvana Soto-Alvear, Ariel Orellana, Francisca Blanco-Herrera

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.

Original languageEnglish
Article number1081
JournalFrontiers in Plant Science
Volume7
Issue numberJULY2016
DOIs
Publication statusPublished - 26 Jul 2016

Fingerprint

root hairs
cell wall components
plant growth-promoting rhizobacteria
polysaccharides
proteins
cell walls
systemic acquired resistance
environmental factors
rhizosphere bacteria
endocytosis
water uptake
nutrient uptake
recycling
proline
physiology
plastics
mutants
receptors
pathogens
infection

Keywords

  • Cell wall
  • Induced systemic resistance
  • Plant growth promoting rhizobacterium
  • PRP3
  • Rhizobacteria
  • Syntaxin
  • Systemic acquired resistance
  • Trafficking

ASJC Scopus subject areas

  • Plant Science

Cite this

Rodriguez-Furlán, Cecilia ; Salinas-Grenet, Hernán ; Sandoval, Omar ; Recabarren, Camilo ; Arraño-Salinas, Paulina ; Soto-Alvear, Sylvana ; Orellana, Ariel ; Blanco-Herrera, Francisca. / The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance. In: Frontiers in Plant Science. 2016 ; Vol. 7, No. JULY2016.
@article{5db5fdb263964aa3aba9e87e6e254d53,
title = "The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance",
abstract = "Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.",
keywords = "Cell wall, Induced systemic resistance, Plant growth promoting rhizobacterium, PRP3, Rhizobacteria, Syntaxin, Systemic acquired resistance, Trafficking",
author = "Cecilia Rodriguez-Furl{\'a}n and Hern{\'a}n Salinas-Grenet and Omar Sandoval and Camilo Recabarren and Paulina Arra{\~n}o-Salinas and Sylvana Soto-Alvear and Ariel Orellana and Francisca Blanco-Herrera",
year = "2016",
month = "7",
day = "26",
doi = "10.3389/fpls.2016.01081",
language = "English",
volume = "7",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A.",
number = "JULY2016",

}

The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance. / Rodriguez-Furlán, Cecilia; Salinas-Grenet, Hernán; Sandoval, Omar; Recabarren, Camilo; Arraño-Salinas, Paulina; Soto-Alvear, Sylvana; Orellana, Ariel; Blanco-Herrera, Francisca.

In: Frontiers in Plant Science, Vol. 7, No. JULY2016, 1081, 26.07.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The root hair specific SYP123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance

AU - Rodriguez-Furlán, Cecilia

AU - Salinas-Grenet, Hernán

AU - Sandoval, Omar

AU - Recabarren, Camilo

AU - Arraño-Salinas, Paulina

AU - Soto-Alvear, Sylvana

AU - Orellana, Ariel

AU - Blanco-Herrera, Francisca

PY - 2016/7/26

Y1 - 2016/7/26

N2 - Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.

AB - Root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family, SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.

KW - Cell wall

KW - Induced systemic resistance

KW - Plant growth promoting rhizobacterium

KW - PRP3

KW - Rhizobacteria

KW - Syntaxin

KW - Systemic acquired resistance

KW - Trafficking

UR - http://www.scopus.com/inward/record.url?scp=84979656428&partnerID=8YFLogxK

U2 - 10.3389/fpls.2016.01081

DO - 10.3389/fpls.2016.01081

M3 - Article

VL - 7

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

IS - JULY2016

M1 - 1081

ER -