The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

Bertsy Goić; Juanita Bustamante; Alvaro Miquel; Marco Alvarez; M. Ines Vera; Pablo D T Valenzuela; Luis O. Burzio

doi:10.1016/j.virol.2008.05.036

The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

Bertsy Goić, Juanita Bustamante, Alvaro Miquel, Marco Alvarez, M. Ines Vera, Pablo D T Valenzuela, Luis O. Burzio

Life Sciences School

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

Original language	English
Pages (from-to)	55-63
Number of pages	9
Journal	Virology
Volume	379
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2008.05.036
Publication status	Published - 15 Sept 2008

Keywords

ISAV
In situ hybridization
Nucleoli
Nucleolin
Nucleoprotein

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2008.05.036

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title = "The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells",

abstract = "The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.",

keywords = "ISAV, In situ hybridization, Nucleoli, Nucleolin, Nucleoprotein",

author = "Bertsy Goi{\'c} and Juanita Bustamante and Alvaro Miquel and Marco Alvarez and Vera, {M. Ines} and Valenzuela, {Pablo D T} and Burzio, {Luis O.}",

note = "Funding Information: The authors wish to thank Micro Technologies Inc., New Brunswick, Canada for providing the ISAV isolate used in this study. We thank Drs. Juan Kuznar, Bernardita Mendez, Arturo Yudelevich and Mario Rosemblatt for their continuous support and enriched discussion. This work was partially supported by Millennium Scientific Initiative No. P-77-09F, Chile, Grants DID-032-03, DID-26-04, DID-57-04 of the Universidad Andr{\'e}s Bello and Grant COPEC-UC No. PC036.",

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TY - JOUR

T1 - The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

AU - Goić, Bertsy

AU - Bustamante, Juanita

AU - Miquel, Alvaro

AU - Alvarez, Marco

AU - Vera, M. Ines

AU - Valenzuela, Pablo D T

AU - Burzio, Luis O.

N1 - Funding Information: The authors wish to thank Micro Technologies Inc., New Brunswick, Canada for providing the ISAV isolate used in this study. We thank Drs. Juan Kuznar, Bernardita Mendez, Arturo Yudelevich and Mario Rosemblatt for their continuous support and enriched discussion. This work was partially supported by Millennium Scientific Initiative No. P-77-09F, Chile, Grants DID-032-03, DID-26-04, DID-57-04 of the Universidad Andrés Bello and Grant COPEC-UC No. PC036.

PY - 2008/9/15

Y1 - 2008/9/15

N2 - The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

AB - The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

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KW - In situ hybridization

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KW - Nucleolin

KW - Nucleoprotein

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The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

Abstract

Keywords

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