The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

Bertsy Goić, Juanita Bustamante, Alvaro Miquel, Marco Alvarez, M. Ines Vera, Pablo D T Valenzuela, Luis O. Burzio

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalVirology
Volume379
Issue number1
DOIs
Publication statusPublished - 15 Sep 2008

Keywords

  • ISAV
  • In situ hybridization
  • Nucleoli
  • Nucleolin
  • Nucleoprotein

ASJC Scopus subject areas

  • Virology

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