Cyanoethylation of formylmethionine tRNA at 60° resulted in a rapid pseudofirst order inactivation of acceptor activity (T 1 2, 28 min). Under the conditions chosen, only the Ψrd in the T-Ψ-C-G loop should have reacted. Absorbancy-temperature profiles of 94% inactivated tRNA revealed a marked decrease in ordered structure which is probably responsible for the loss of activity.
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 6 Jan 1970|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology