The function of pseudouridylic acid in transfer RNA. III. Inactivation of formylmethionine transfer RNA of E. coli by cyanoethylation with acrylonitrile

M. A Q Siddiqui, Manuel Krauskopf, James Ofengand

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Cyanoethylation of formylmethionine tRNA at 60° resulted in a rapid pseudofirst order inactivation of acceptor activity (T 1 2, 28 min). Under the conditions chosen, only the Ψrd in the T-Ψ-C-G loop should have reacted. Absorbancy-temperature profiles of 94% inactivated tRNA revealed a marked decrease in ordered structure which is probably responsible for the loss of activity.

Original languageEnglish
Pages (from-to)156-164
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume38
Issue number1
DOIs
Publication statusPublished - 6 Jan 1970

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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