The expression of the bstVIM gene from Bacillus stearothermophilus V is restricted to vegetative cell growth

E. Gonzalez, C. Padilla, C. Saavedra, C. Vasquez

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The activity of BstVI DNA methyltransferase was monitored during the sporulative cycle of Bacillus stearothermophilus V. Significant methylase activity was found only in bacteria growing vegetatively. This was confirmed by Northern hybridization, which indicated that the bstVIM gene was not transcribed in cells undergoing sporulation. Supporting evidence came from experiments which demonstrated that the RNA polymerase holoenzyme from these cells did not recognize the promoter elements upstream of the bstVIM gene.

Original languageEnglish
Pages (from-to)1337-1340
Number of pages4
JournalMicrobiology
Volume140
Issue number6
DOIs
Publication statusPublished - 1994

Keywords

  • Bacillus stearothermophilus
  • Differential gene expression
  • Sporulation
  • Thermophilic promoters

ASJC Scopus subject areas

  • Microbiology

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