The endophytic fungus Chaetomium cupreum regulates expression of genes involved in the tolerance to metals and plant growth promotion in Eucalyptus globulus roots

Javier Ortiz, Javiera Soto, Alejandra Fuentes, Héctor Herrera, Claudio Meneses, César Arriagada

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The endophytic strain Chaetomium cupreum isolated from metal-contaminated soil was inoculated in Eucalyptus globulus roots to identify genes involved in metal stress response and plant growth promotion. We analyzed the transcriptome of E. globulus roots inoculated with C. cupreum. De novo sequencing, assembly, and analysis were performed to identify molecular mechanisms involved in metal stress tolerance and plant growth promotion. A total of 393,371,743 paired-end reads were assembled into 135,155 putative transcripts. It was found that 663 genes significantly changed their expression in the presence of treatment, of which 369 were up-regulated and 294 were down-regulated. We found differentially expressed genes (DEGs) encoding metal transporters, transcription factors, stress and defense response proteins, as well as DEGs involved in auxin biosynthesis and metabolism. Our results showed that the inoculation of C. cupreum enhanced tolerance to metals and growth promotion on E. globulus. This study provides new information to understand molecular mechanisms involved in plant–microbe interactions under metals stress.

Original languageEnglish
Article number490
JournalMicroorganisms
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 2019

Keywords

  • Chaetomium cupreum
  • Endophyte
  • Eucalyptus globulus roots
  • Metal stress
  • Transcriptome

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

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