The crystal structure of triosephosphate isomerase (TIM) from Thermotoga maritima: A comparative thermostability structural analysis of ten different TIM structures

Dominique Maes, Johan P. Zeelen, Narmada Thanki, Nicola Beaucamp, Marco Alvarez, Thi Minh Hoa Dao, Jan Backmann, Joseph A. Martial, Lode Wyns, Rainer Jaenicke, Rik K. Wierenga

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)

Abstract

The molecular mechanisms that evolution has been employing to adapt to environmental temperatures are poorly understood. To gain some further insight into this subject we solved the crystal structure of triosephosphate isomerase (TIM) from the hyperthermophilic bacterium Thermotoga maritima (TmTIM). The enzyme is a tetramer, assembled as a dimer of dimers, suggesting that the tetrameric wild-type phosphoglycerate kinase PGK-TIM fusion protein consists of a core of two TIM dimers covalently linked to 4 PGK units. The crystal structure of TmTIM represents the most thermostable TIM presently known in its 3D-structure. It adds to a series of nine known TIM structures from a wide variety of organisms, spanning the range from psychrophiles to hyperthermophiles. Several properties believed to be involved in the adaptation to different temperatures were calculated and compared for all ten structures. No sequence preferences, correlated with thermal stability, were apparent from the amino acid composition or from the analysis of the loops and secondary structure elements of the ten TIMs. A common feature for both psychrophilic and T. maritima TIM is the large number of salt bridges compared with the number found in mesophilic TIMs. In the two thermophilic TIMs, the highest amount of accessible hydrophobic surface is buried during the folding and assembly process.

Original languageEnglish
Pages (from-to)441-453
Number of pages13
JournalProteins: Structure, Function and Genetics
Volume37
Issue number3
DOIs
Publication statusPublished - 15 Nov 1999

Keywords

  • Hydrophobicity
  • Hyperthermophile
  • Psychrophile
  • Salt bridges
  • Thermophile

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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