Abstract
1α,25-dihydroxy vitamin D3 has a major role in the regulation of the bone metabolism as it promotes the expression of key bone-related proteins in osteoblastic cells. In recent years it has become increasingly evident that in addition to its well-established genomic actions, 1α,25-dihydroxy vitamin D3 induces non-genomic responses by acting through a specific plasma membrane-associated receptor. Results from several groups suggest that the classical nuclear 1α,25-dihydroxy vitamin D3 receptor (VDR) is also responsible for these non-genomic actions of 1α,25-dihydroxy vitamin D3. Here, we have used siRNA to suppress the expression of VDR in osteoblastic cells and assessed the role of VDR in the non-genomic response to 1α,25-dihydroxy vitamin D3. We report that expression of the classic VDR in osteoblasts is required to generate a rapid 1α,25-dihydroxy vitamin D3-mediated increase in the intracellular Ca 2+ concentration, a hallmark of the non-genomic actions of 1α,25-dihydroxy vitamin D3 in these cells.
Original language | English |
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Pages (from-to) | 995-1000 |
Number of pages | 6 |
Journal | Journal of Cellular Biochemistry |
Volume | 99 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Nov 2006 |
Keywords
- Non-genomic actions of vitamin D3
- Osteoblastic cells
- Vitamin D3 receptor
ASJC Scopus subject areas
- Biochemistry
- Cell Biology