The classic receptor for 1α,25-dihydroxy vitamin D3 is required for non-genomic actions of 1α,25-dihydroxy vitamin D3 in osteosarcoma cells

Soraya Bravo, Roberto Paredes, Pamela Izaurieta, Jane B. Lian, Janet L. Stein, Gary S. Stein, Maria Victoria Hinrichs, Juan Olate, Luis G. Aguayo, Martin Montecino

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

1α,25-dihydroxy vitamin D3 has a major role in the regulation of the bone metabolism as it promotes the expression of key bone-related proteins in osteoblastic cells. In recent years it has become increasingly evident that in addition to its well-established genomic actions, 1α,25-dihydroxy vitamin D3 induces non-genomic responses by acting through a specific plasma membrane-associated receptor. Results from several groups suggest that the classical nuclear 1α,25-dihydroxy vitamin D3 receptor (VDR) is also responsible for these non-genomic actions of 1α,25-dihydroxy vitamin D3. Here, we have used siRNA to suppress the expression of VDR in osteoblastic cells and assessed the role of VDR in the non-genomic response to 1α,25-dihydroxy vitamin D3. We report that expression of the classic VDR in osteoblasts is required to generate a rapid 1α,25-dihydroxy vitamin D3-mediated increase in the intracellular Ca 2+ concentration, a hallmark of the non-genomic actions of 1α,25-dihydroxy vitamin D3 in these cells.

Original languageEnglish
Pages (from-to)995-1000
Number of pages6
JournalJournal of Cellular Biochemistry
Volume99
Issue number4
DOIs
Publication statusPublished - 1 Nov 2006

Keywords

  • Non-genomic actions of vitamin D3
  • Osteoblastic cells
  • Vitamin D3 receptor

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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