The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten; Berit Ebert; Lifeng Liu; Solomon Stonebloom; Andreia M. Smith-Moritz; Markus Pauly; Ariel Orellana; Henrik Vibe Scheller; Joshua L. Heazlewood

doi:10.1038/ncomms12119

The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten, Berit Ebert, Lifeng Liu, Solomon Stonebloom, Andreia M. Smith-Moritz, Markus Pauly, Ariel Orellana, Henrik Vibe Scheller, Joshua L. Heazlewood

Life Sciences School

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53 Citations (Scopus)

Abstract

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

Original language	English
Article number	12119
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12119
Publication status	Published - 6 Jul 2016

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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title = "The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development",

abstract = "Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.",

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AU - Rautengarten, Carsten

AU - Ebert, Berit

AU - Liu, Lifeng

AU - Stonebloom, Solomon

AU - Smith-Moritz, Andreia M.

AU - Pauly, Markus

AU - Orellana, Ariel

AU - Scheller, Henrik Vibe

AU - Heazlewood, Joshua L.

PY - 2016/7/6

Y1 - 2016/7/6

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AB - Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

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The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Abstract

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