The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten, Berit Ebert, Lifeng Liu, Solomon Stonebloom, Andreia M. Smith-Moritz, Markus Pauly, Ariel Orellana, Henrik Vibe Scheller, Joshua L. Heazlewood

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

Original languageEnglish
Article number12119
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 6 Jul 2016

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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