Tenacibaculum piscium Sp. Nov., isolated from skin ulcers of sea-farmed fish, and description of tenacibaculum finnmarkense Sp. nov. with subdivision into genomovars finnmarkense and ulcerans

Anne Berit Olsen, Bjørn Spilsberg, Hanne K. Nilsen, Karin Lagesen, Snorre Gulla, Ruben Avendaño-Herrera, Rute Irgang, Eric Duchaud, Duncan J. Colquhoun

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Results of previous multilocus sequence and whole-genome-based analyses have suggested that a homogeneous group of isolates belonging to the genus Tenacibaculum, represented by strain TNO020T and associated with skin ulcer development in sea-farmed fish, represents an as-yet-undescribed species. Comparative whole-genome analysis performed in the present study clustered five isolates, including TNO020T, in a distinct lineage within the genus Tenacibaculum. Phenotypic differences, high intra-cluster average nucleotide identity (ANI) values and low ANI values with other Tenacibaculum species support the proposal of a novel species, for which we propose the name Tenacibaculum piscium sp. nov. with strain TNO020T (=CCUG 73833T=NCIMB 15240T) as the type strain. Further, large-scale genome analyses confirmed the existence of two different phylogenetic lineages within ‘T. finnmarkense’, a species effectively but not validly published previously. ANI values just above the species delineation threshold of 95–96% confirmed that both lineages belong to the same species. This result was also supported by DNA–DNA hybridization values. Phenotypically, the two conspecific lineages are distinguishable by differences in growth temperature range and ability to degrade l-proline. For the group of isolates already commonly known as ‘T. finnmarkense’, we propose the name Tenacibaculum finnmarkense sp. nov., with strain TNO006T (=CCUG 73831T=NCIMB 15238T) as the type strain. We further propose the subdivision of T. finnmarkense sp. nov. into two genomovars, T. finnmarkense genomovar finnmarkense with strain TNO006T (=CCUG 73831T=NCIMB 15238T) as the type strain and T. finnmarkense genomovar ulcerans with strain TNO010T (=CCUG 73832T=NCIMB 15239T) as the type strain.

Original languageEnglish
Article number004501
Pages (from-to)6079-6090
Number of pages12
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number12
DOIs
Publication statusPublished - 2020

Keywords

  • CgMLST
  • Tenacibaculosis
  • Tenacibaculum finnmarkense
  • Tenacibaculum finnmarkense genomovar finnmarkense
  • Tenacibaculum finnmarkense genomovar ulcerans
  • Tenacibaculum piscium

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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