TY - JOUR
T1 - Temporal and spatial expression pattern of the myostatin gene during larval and juvenile stages of the Chilean flounder (Paralichthys adspersus)
AU - Delgado, Iselys
AU - Fuentes, Eduardo
AU - Escobar, Sebastián
AU - Navarro, Cristina
AU - Corbeaux, Tatiana
AU - Reyes, Ariel E.
AU - Vera, María Inés
AU - Álvarez, Marco
AU - Molina, Alfredo
N1 - Funding Information:
This work was supported by Grants N°1050272 from the FONDECYT and 15-03/28-04/13-06I from the UNAB Research Fund to A.M and FONDECYT N°1060441 to A.E.R. We would like to thank Dr. Manuel Krauskopf for critical reading of the manuscript. We thank Juan Manuel Estrada for technical assistance and animal care in the Centro de Investigación Marina de Quintay (CIMARQ).
PY - 2008/10
Y1 - 2008/10
N2 - The full length cDNA sequence of the myostatin gene was cloned from a teleostean fish, the Chilean flounder (Paralichthys adspersus) through RT-PCR amplification coupled with the RACE approach to complete the 5′- and 3′-region. The deduced amino acid sequence encodes a protein of 377 amino acid residues, including the structural domains responsible for its biological activity. Amino acid sequence comparison revealed high sequence conservation, and confirmed that the isolated sequence corresponds to the MSTN1 gene. Gene expression analysis showed that cfMSTN mRNA is present in a wide variety of tissues in juvenile fish. In addition, we assessed the spatial expression pattern of the MSTN mRNA during embryos and larval stages through whole mount in situ hybridization. No expression was observed in embryos, whereas in larvae of 8 and 9 days post fertilization, the notochord, somites, intestine and some discrete territories in the head, such as brain and eye, were positive for MSTN mRNA. Our results contribute to the knowledge of the MSTN system in larval and juvenile stages; in particular the strong expression observed in the notochord suggests that MSTN, in synchronization with positive growth signals, may play an important role in the control of the development of larvae somites.
AB - The full length cDNA sequence of the myostatin gene was cloned from a teleostean fish, the Chilean flounder (Paralichthys adspersus) through RT-PCR amplification coupled with the RACE approach to complete the 5′- and 3′-region. The deduced amino acid sequence encodes a protein of 377 amino acid residues, including the structural domains responsible for its biological activity. Amino acid sequence comparison revealed high sequence conservation, and confirmed that the isolated sequence corresponds to the MSTN1 gene. Gene expression analysis showed that cfMSTN mRNA is present in a wide variety of tissues in juvenile fish. In addition, we assessed the spatial expression pattern of the MSTN mRNA during embryos and larval stages through whole mount in situ hybridization. No expression was observed in embryos, whereas in larvae of 8 and 9 days post fertilization, the notochord, somites, intestine and some discrete territories in the head, such as brain and eye, were positive for MSTN mRNA. Our results contribute to the knowledge of the MSTN system in larval and juvenile stages; in particular the strong expression observed in the notochord suggests that MSTN, in synchronization with positive growth signals, may play an important role in the control of the development of larvae somites.
KW - Chilean flounder
KW - Fish
KW - Muscle growth
KW - Myostatin
KW - Whole mount in situ hybridization
UR - http://www.scopus.com/inward/record.url?scp=50049124696&partnerID=8YFLogxK
U2 - 10.1016/j.cbpb.2008.07.003
DO - 10.1016/j.cbpb.2008.07.003
M3 - Article
C2 - 18674631
AN - SCOPUS:50049124696
SN - 1096-4959
VL - 151
SP - 197
EP - 202
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 2
ER -