Taxonomic reassessment of N4-like viruses using comparative genomics and proteomics suggests a new subfamily - “Enquartavirinae”

Johannes Wittmann, Jochen Klumpp, Andrea I. Moreno Switt, Abdelbaset Yagubi, Hans Wolfgang Ackermann, Martin Wiedmann, Antonet Svircev, John H E Nash, Andrew M. Kropinski

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21 Citations (Scopus)

Abstract

The GenBank database currently contains sequence data for 33 N4-like viruses, with only one, Escherichia phage N4, being formally recognized by the ICTV. The genus N4likevirus is uniquely characterized by that fact that its members possess an extremely large, virion-associated RNA polymerase. Using a variety of proteomic, genomic and phylogenetic tools, we have demonstrated that the N4-like phages are not monophyletic and that N4 is actually a genomic orphan. We propose to create four new genera: “G7cvirus” (consisting of phages G7C, IME11, KBNP21, vB_EcoP_PhAPEC5, vB_EcoP_PhAPEC7, Bp4, EC1-UPM and pSb-1), “Lit1virus” (LIT1, PA26 and vB_PaeP_C2-10_Ab09), “Sp58virus” (SP058 and SP076), and “Dss3virus” (DSS3φ2 and EE36φ1). We propose that coliphage N4, the members of “G7cvirus”, Erwinia phage Ea9-2, and Achromobacter phage JWAlpha should be considered members of the same subfamily, which we tentatively call the “Enquartavirinae”.

Original languageEnglish
Pages (from-to)3053-3062
Number of pages10
JournalArchives of Virology
Volume160
Issue number12
DOIs
Publication statusPublished - 23 Sep 2015

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

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    Wittmann, J., Klumpp, J., Moreno Switt, A. I., Yagubi, A., Ackermann, H. W., Wiedmann, M., Svircev, A., Nash, J. H. E., & Kropinski, A. M. (2015). Taxonomic reassessment of N4-like viruses using comparative genomics and proteomics suggests a new subfamily - “Enquartavirinae”. Archives of Virology, 160(12), 3053-3062. https://doi.org/10.1007/s00705-015-2609-6