Systemic oxidative stress and endothelial dysfunction is associated with an attenuated acute vascular response to inhaled prostanoid in pulmonary artery hypertension patients

Luigi A. Gabrielli, Pablo F. Castro, Ivan Godoy, Rosemarie Mellado, Robert C. Bourge, Hernan Alcaino, Mario Chiong, Douglas Greig, Hugo E. Verdejo, Mario Navarro, Rafael Lopez, Barbra Toro, Clara Quiroga, Guillermo Díaz-Araya, Sergio Lavandero, Lorena Garcia

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Background: Systemic endothelial dysfunction and increased oxidative stress have been observed in pulmonary arterial hypertension (PAH). We evaluate whether oxidative stress and endothelial dysfunction are associated with acute pulmonary vascular bed response to an inhaled prostanoid in PAH patients. Methods: Fourteen idiopathic PAH patients and 14 controls were included. Oxidative stress was assessed through plasma malondialdehyde (MDA) levels and xanthine oxidase (XO) and endothelial-bound superoxide dismutase (eSOD) activity. Brachial artery endothelial-dependent flow-mediated vasodilation (FMD) was used to evaluate endothelial function. Hemodynamic response to inhaled iloprost was assessed with transthoracic echocardiography. Results: PAH patients showed impaired FMD (2.8 ± 0.6 vs. 10.7 ± 0.6%, P <.01), increased MDA levels and XO activity (0.6 ± 0.2 vs. 0.3 ± 0.2 μM, P <.01 and 0.04 ± 0.01 vs. 0.03 ± 0.01 U/mL, P =.02, respectively) and decreased eSOD activity (235 ± 23 vs. 461 ± 33 AUC, P <.01). Iloprost improved right cardiac output (3.7 ± 0.6 to 4.1 ± 1.2 L/min, P =.02) and decreased pulmonary vascular resistance (4.1 ± 1.1 to 2.9 ± 0.9 Wood U, P =.01). Changes in right cardiac output after prostanoid inhalation correlated significantly with baseline eSOD activity and FMD (Rho: 0.61, P <.01 and Rho: 0.63, P =.01, respectively). Conclusion: PAH patients show increased systemic oxidative stress and endothelial dysfunction markers. Response to inhaled prostanoid is inversely related to both parameters.

Original languageEnglish
Pages (from-to)1012-1017
Number of pages6
JournalJournal of Cardiac Failure
Volume17
Issue number12
DOIs
Publication statusPublished - 1 Dec 2011

Keywords

  • endothelial function
  • endothelial superoxide dismutase
  • oxidative stress
  • Pulmonary hypertension

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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