Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

Pablo Valenzuela; Angelica Medina; William J. Rutter; Gustav Ammerer; Benjamin D. Hall

doi:10.1038/298347a0

Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

Pablo Valenzuela, Angelica Medina, William J. Rutter, Gustav Ammerer, Benjamin D. Hall

Life Sciences School

Research output: Contribution to journal › Article › peer-review

650 Citations (Scopus)

Abstract

The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

Original language	English
Pages (from-to)	347-350
Number of pages	4
Journal	Nature
Volume	298
Issue number	5872
DOIs	https://doi.org/10.1038/298347a0
Publication status	Published - 1982

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10.1038/298347a0

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title = "Synthesis and assembly of hepatitis B virus surface antigen particles in yeast",

abstract = "The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.",

author = "Pablo Valenzuela and Angelica Medina and Rutter, {William J.} and Gustav Ammerer and Hall, {Benjamin D.}",

year = "1982",

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volume = "298",

pages = "347--350",

journal = "Nature",

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T1 - Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

AU - Valenzuela, Pablo

AU - Medina, Angelica

AU - Rutter, William J.

AU - Ammerer, Gustav

AU - Hall, Benjamin D.

PY - 1982

Y1 - 1982

N2 - The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

AB - The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

UR - http://www.scopus.com/inward/record.url?scp=0019985965&partnerID=8YFLogxK

U2 - 10.1038/298347a0

DO - 10.1038/298347a0

M3 - Article

C2 - 7045698

AN - SCOPUS:0019985965

SN - 0028-0836

VL - 298

SP - 347

EP - 350

JO - Nature

JF - Nature

IS - 5872

ER -

Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

Abstract

ASJC Scopus subject areas

UN SDGs

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