Synthesis and assembly of a functionally active recombinant platelet-derived growth factor AB heterodimer

A. Ostman, L. Rall, A. Hammacher, M. A. Wormstead, D. Coit, P. Valenzuela, C. Betsholtz, B. Westermark, C. H. Heldin

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62 Citations (Scopus)

Abstract

A Chinese hamster ovary cell line that stably expresses transfected human platelet-derived growth factor (PDGF) A and B chain precursors was established. All three dimeric combinations of PDGF chains were produced by this cell line; their biosynthesis, assembly, and processing were followed by pulse-chase analyses. PDGF-AA, PDGF-AB, and PDGF-BB were processed to M(r) values of about 30,000 and were accumulated in these forms in the medium. In addition, PDGF-BB was further processed to a 24-kDa component, which remained cell-associated. The major secreted component was PDGF-AB, which was purified and shown to have structural and functional characteristics indistinguishable from PDGF-AB purified from human platelets.

Original languageEnglish
Pages (from-to)16202-16208
Number of pages7
JournalJournal of Biological Chemistry
Volume263
Issue number31
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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