Subnuclear localization and intranuclear trafficking of transcription factors.

Sayyed K. Zaidi; Ricardo F. Medina; Shirwin M. Pockwinse; Rachit Bakshi; Krishna P. Kota; Syed A. Ali; Daniel W. Young; Jeffrey A. Nickerson; Amjad Javed; Martin Montecino; Andre J. van Wijnen; Jane B. Lian; Janet L. Stein; Gary S. Stein

Subnuclear localization and intranuclear trafficking of transcription factors.

Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffrey A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Life Sciences School

Research output: Contribution to journal › Article › peer-review

Abstract

Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original language	English
Pages (from-to)	77-93
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	647
Publication status	Published - 2010

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Subnuclear localization and intranuclear trafficking of transcription factors.",

abstract = "Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.",

author = "Zaidi, {Sayyed K.} and Medina, {Ricardo F.} and Pockwinse, {Shirwin M.} and Rachit Bakshi and Kota, {Krishna P.} and Ali, {Syed A.} and Young, {Daniel W.} and Nickerson, {Jeffrey A.} and Amjad Javed and Martin Montecino and {van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.}",

year = "2010",

language = "English",

volume = "647",

pages = "77--93",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

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TY - JOUR

T1 - Subnuclear localization and intranuclear trafficking of transcription factors.

AU - Zaidi, Sayyed K.

AU - Medina, Ricardo F.

AU - Pockwinse, Shirwin M.

AU - Bakshi, Rachit

AU - Kota, Krishna P.

AU - Ali, Syed A.

AU - Young, Daniel W.

AU - Nickerson, Jeffrey A.

AU - Javed, Amjad

AU - Montecino, Martin

AU - van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - Stein, Gary S.

PY - 2010

Y1 - 2010

N2 - Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

AB - Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

UR - http://www.scopus.com/inward/record.url?scp=79952109378&partnerID=8YFLogxK

M3 - Article

C2 - 20694661

SN - 1064-3745

VL - 647

SP - 77

EP - 93

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Subnuclear localization and intranuclear trafficking of transcription factors.

Abstract

ASJC Scopus subject areas

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